CDK8 is associated with RAP2.6 and SnRK2.6 and positively modulates abscisic acid signaling and drought response inArabidopsis
文献类型：期刊论文
| 作者 | Zhu, Yingfang3; Huang, Pengcheng3; Guo, Pengcheng3; Chong, Leelyn3; Yu, Gaobo2,8; Sun, Xiaoli2,8; Hu, Tao4,8; Li, Yuan1,6; Hsu, Chuan-Chih7; Tang, Kai6,8 |
| 刊名 | NEW PHYTOLOGIST
 |
| 出版日期 | 2020-08-02 |
| 页码 | 18 |
| 关键词 | ABA-responsive genes abiotic stress responses Arabidopsis CDK8 Mediator complex RAP2 6 SnRK2 6 |
| ISSN号 | 0028-646X |
| DOI | 10.1111/nph.16787 |
| 英文摘要 | CDK8 is a key subunit of Mediator complex, a large multiprotein complex that is a fundamental part of the conserved eukaryotic transcriptional machinery. However, the biological functions ofCDK8in plant abiotic stress responses remain largely unexplored. Here, we demonstratedCDK8as a critical regulator in the abscisic acid (ABA) signaling and drought response pathways inArabidopsis. Compared to wild-type,cdk8mutants showed reduced sensitivity to ABA, impaired stomatal apertures and hypersensitivity to drought stress. Transcriptomic and chromatin immunoprecipitation analysis revealed thatCDK8positively regulates the transcription of several ABA-responsive genes, probably through promoting the recruitment of RNA polymerase II to their promoters. We discovered that both CDK8 and SnRK2.6 interact physically with an ERF/AP2 transcription factor RAP2.6, which can directly bind to the promoters ofRD29AandCOLD-REGULATED 15A(COR15A) with GCC or DRE elements, thereby promoting their expression. Importantly, we also showed thatCDK8is essential for the ABA-induced expression ofRAP2.6andRAP2.6-mediated upregulation of ABA-responsive genes, indicating that CDK8 could link the SnRK2.6-mediated ABA signaling to RNA polymerase II to promote immediate transcriptional response to ABA and drought signals. Overall, our data provide new insights into the roles of CDK8 in modulating ABA signaling and drought responses. |
| 资助项目 | National Natural Science Foundation of China[NSFC 31900238] ; Chinese Academy of Sciences grant |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000554398700001 |
| 出版者 | WILEY |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/15534]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Zhu, Yingfang |
| 作者单位 | 1.China Agr Univ, Coll Biol Sci, State Key Lab Plant Physiol & Biochem, Beijing 100193, Peoples R China 2.Heilongjiang Bayi Agr Univ, Crop Stress Mol Biol Lab, Daqing 163711, Peoples R China 3.Henan Univ, Sch Life Sci, Key Lab Cotton Biol, State Key Lab Crop Stress Adaptat & Improvement, Kaifeng 475001, Peoples R China 4.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Peoples R China 5.Purdue Univ, Dept Bot & Plant Pathol, W Lafayette, IN 47907 USA 6.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai Ctr Plant Stress Biol, Shanghai 200032, Peoples R China 7.Purdue Univ, Dept Biochem, W Lafayette, IN 47907 USA 8.Purdue Univ, Dept Hort & Landscape Architecture, W Lafayette, IN 47907 USA |
| 推荐引用方式 GB/T 7714 | Zhu, Yingfang,Huang, Pengcheng,Guo, Pengcheng,et al. CDK8 is associated with RAP2.6 and SnRK2.6 and positively modulates abscisic acid signaling and drought response inArabidopsis[J]. NEW PHYTOLOGIST,2020:18. |
| APA | Zhu, Yingfang.,Huang, Pengcheng.,Guo, Pengcheng.,Chong, Leelyn.,Yu, Gaobo.,...&Zhu, Jian-Kang.(2020).CDK8 is associated with RAP2.6 and SnRK2.6 and positively modulates abscisic acid signaling and drought response inArabidopsis.NEW PHYTOLOGIST,18. |
| MLA | Zhu, Yingfang,et al."CDK8 is associated with RAP2.6 and SnRK2.6 and positively modulates abscisic acid signaling and drought response inArabidopsis".NEW PHYTOLOGIST (2020):18. |
入库方式： OAI收割
来源：武汉植物园
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