A GATA Transcription Factor from Soybean (Glycine max) Regulates Chlorophyll Biosynthesis and Suppresses Growth in the Transgenic Arabidopsis thaliana
文献类型：期刊论文
| 作者 | Zhang, Chanjuan3; Huang, Yi3; Xiao, Zhiyuan3; Yang, Hongli3; Hao, Qingnan3; Yuan, Songli3; Chen, Haifeng3; Chen, Limiao3; Chen, Shuilian3; Zhou, Xinan3 |
| 刊名 | PLANTS-BASEL
 |
| 出版日期 | 2020-08-01 |
| 卷号 | 9期号:8页码:16 |
| 关键词 | Glycine max soybean GATA transcription factor chlorophyll photosynthesis growth nitrogen |
| DOI | 10.3390/plants9081036 |
| 英文摘要 | Chlorophyll plays an essential role in photosynthetic light harvesting and energy transduction in green tissues of higher plants and is closely related to photosynthesis and crop yield. Identification of transcription factors (TFs) involved in regulating chlorophyll biosynthesis is still limited in soybean (Glycine max), and the previously identifiedGmGATA58is suggested to potentially modulate chlorophyll and nitrogen metabolisms, but its complete function is still unknown. In this study, subcellular localization assay showed that GmGATA58 was localized in the nucleus. Histochemical GUS assay and qPCR assay indicated thatGmGATA58was mainly expressed in leaves and responded to nitrogen, light and phytohormone treatments. Overexpression ofGmGATA58in theArabidopsis thalianaorthologAtGATA21(gnc) mutant complemented the greening defect, while overexpression in Arabidopsis wild-type led to increasing chlorophyll content in leaves through up-regulating the expression levels of the large of chlorophyll biosynthetic pathway genes, but suppressing plant growth and yield, although the net photosynthetic rate was slightly improved. Dual-luciferase reporter assay also supported that GmGATA58 activated the transcription activities of three promoters of key chlorophyll biosynthetic genes of soybean in transformed protoplast of Arabidopsis. It is concluded thatGmGATA58played an important role in regulating chlorophyll biosynthesis, but suppressed plant growth and yield in transgenic Arabidopsis. |
| 资助项目 | National Transgenic Project[2016ZX08004-005] ; National Key R&D Program of China[2017YFD0101500] |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000572559000001 |
| 出版者 | MDPI |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/15733]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Zhou, Xinan; Huang, Wenjun |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan Bot Garden, Wuhan 430074, Peoples R China 2.Chinese Acad Sci, Innovat Acad Seed Design, Beijing 100101, Peoples R China 3.Chinese Acad Agr Sci, Key Lab Biol & Genet Improvement Oil Crops, Minist Agr & Rural Affairs, Oil Crops Res Inst, Wuhan 430062, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Chanjuan,Huang, Yi,Xiao, Zhiyuan,et al. A GATA Transcription Factor from Soybean (Glycine max) Regulates Chlorophyll Biosynthesis and Suppresses Growth in the Transgenic Arabidopsis thaliana[J]. PLANTS-BASEL,2020,9(8):16. |
| APA | Zhang, Chanjuan.,Huang, Yi.,Xiao, Zhiyuan.,Yang, Hongli.,Hao, Qingnan.,...&Huang, Wenjun.(2020).A GATA Transcription Factor from Soybean (Glycine max) Regulates Chlorophyll Biosynthesis and Suppresses Growth in the Transgenic Arabidopsis thaliana.PLANTS-BASEL,9(8),16. |
| MLA | Zhang, Chanjuan,et al."A GATA Transcription Factor from Soybean (Glycine max) Regulates Chlorophyll Biosynthesis and Suppresses Growth in the Transgenic Arabidopsis thaliana".PLANTS-BASEL 9.8(2020):16. |
入库方式： OAI收割
来源：武汉植物园
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