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Lifestyle adaptations of Rhizobium from rhizosphere to symbiosis

文献类型：期刊论文

作者Wheatley, Rachel M.1; Ford, Brandon L.1; Li, Li2; Aroney, Samuel T. N.1; Knights, Hayley E.1; Ledermann, Raphael1; East, Alison K.1; Ramachandran, Vinoy K.1; Poole, Philip S.1
刊名PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
出版日期2020-09-22
卷号117期号:38页码:23823-23834
关键词Rhizobium legume N-2 fixation nodulation rhizosphere
ISSN号0027-8424
DOI10.1073/pnas.2009094117
英文摘要By analyzing successive lifestyle stages of a model Rhizobium-legume symbiosis using mariner-based transposon insertion sequencing (INSeq), we have defined the genes required for rhizosphere growth, root colonization, bacterial infection, N-2-fixing bacteroids, and release from legume (pea) nodules. While only 27 genes are annotated as nif and fix in Rhizobium leguminosarum, we show 603 genetic regions (593 genes, 5 transfer RNAs, and 5 RNA features) are required for the competitive ability to nodulate pea and fix N-2. Of these, 146 are common to rhizosphere growth through to bacteroids. This large number of genes, defined as rhizosphereprogressive, highlights how critical successful competition in the rhizosphere is to subsequent infection and nodulation. As expected, there is also a large group (211) specific for nodule bacteria and bacteroid function. Nodule infection and bacteroid formation require genes for motility, cell envelope restructuring, nodulation signaling, N-2 fixation, and metabolic adaptation. Metabolic adaptation includes urea, erythritol and aldehyde metabolism, glycogen synthesis, dicarboxylate metabolism, and glutamine synthesis (GlnII). There are 17 separate lifestyle adaptations specific to rhizosphere growth and 23 to root colonization, distinct from infection and nodule formation. These results dramatically highlight the importance of competition at multiple stages of a Rhizobium-legume symbiosis.
资助项目Natural Environment Research Council[NE/L501530/1] ; Biotechnology and Biological Sciences Research Council[BB/M011224/1] ; Biotechnology and Biological Sciences Research Council[BB/N013387/1] ; Swiss National Science Foundation Postdoc.Mobility Grant[183901]
WOS研究方向Science & Technology - Other Topics
语种英语
WOS记录号WOS:000575881900004
出版者NATL ACAD SCIENCES
源URL[http://202.127.146.157/handle/2RYDP1HH/15871]  
专题中国科学院武汉植物园
通讯作者Ramachandran, Vinoy K.; Poole, Philip S.
作者单位1.Univ Oxford, Dept Plant Sci, Oxford OX1 3RB, England
2.Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan Bot Garden, Wuhan 430074, Peoples R China
推荐引用方式
GB/T 7714		 Wheatley, Rachel M.,Ford, Brandon L.,Li, Li,et al. Lifestyle adaptations of Rhizobium from rhizosphere to symbiosis[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2020,117(38):23823-23834.
APA Wheatley, Rachel M..,Ford, Brandon L..,Li, Li.,Aroney, Samuel T. N..,Knights, Hayley E..,...&Poole, Philip S..(2020).Lifestyle adaptations of Rhizobium from rhizosphere to symbiosis.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,117(38),23823-23834.
MLA Wheatley, Rachel M.,et al."Lifestyle adaptations of Rhizobium from rhizosphere to symbiosis".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 117.38(2020):23823-23834.

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来源：武汉植物园

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