External alpha-carbonic anhydrase and solute carrier 4 are required for bicarbonate uptake in a freshwater angiosperm
文献类型：期刊论文
| 作者 | Huang, Wenmin1,5; Han, Shijuan2,5; Jiang, Hongsheng5; Gu, Shuping3; Li, Wei5; Gontero, Brigitte1; Maberly, Stephen C.4 |
| 刊名 | JOURNAL OF EXPERIMENTAL BOTANY
 |
| 出版日期 | 2020-10-07 |
| 卷号 | 71期号:19页码:6004-6014 |
| ISSN号 | 0022-0957 |
| 关键词 | Anion exchange protein bicarbonate carbonic anhydrase (CA) CO2-concentrating mechanisms (CCMs) inorganic carbon acquisition Ottelia alismoides pH drift photosynthesis solute carrier 4 (SLC4) |
| DOI | 10.1093/jxb/eraa351 |
| 英文摘要 | The freshwater monocot Ottelia alismoides is the only known species to operate three CO2-concentrating mechanisms (CCMs): constitutive bicarbonate (HCO3-) use, C4 photosynthesis, and facultative Crassulacean acid metabolism, but the mechanism of HCO3- use is unknown. We found that the inhibitor of an anion exchange protein, 4,4'-diisothiocyanatostilbene-2,2'-disulfonate (DIDS), prevented HCO3- use but also had a small effect on CO2 uptake. An inhibitor of external carbonic anhydrase (CA), acetazolamide (AZ), reduced the affinity for CO2 uptake but also prevented HCO3- use via an effect on the anion exchange protein. Analysis of mRNA transcripts identified a homologue of solute carrier 4 (SLC4) responsible for HCO3- transport, likely to be the target of DIDS, and a periplasmic a-carbonic anhydrase 1 (alpha a-CA1). A model to quantify the contribution of the three different pathways involved in inorganic carbon uptake showed that passive CO2 diffusion dominates inorganic carbon uptake at high CO2 concentrations. However, as CO2 concentrations fall, two other pathways become predominant: conversion of HCO3- to CO2 at the plasmalemma by alpha-CA1 and transport of HCO3- across the plasmalemma by SLC4. These mechanisms allow access to a much larger proportion of the inorganic carbon pool and continued photosynthesis during periods of strong carbon depletion in productive ecosystems. |
| 资助项目 | Strategic Priority Research Program of the Chinese Academy of Sciences[XDB31000000] ; Chinese Academy of Sciences President's International Fellowship Initiative[2015VBA023] ; Chinese Academy of Sciences President's International Fellowship Initiative[2016VBA006] ; National Natural Science Foundation of China[31970368] |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| 出版者 | OXFORD UNIV PRESS |
| WOS记录号 | WOS:000577075400025 |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/15890]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Li, Wei; Gontero, Brigitte; Maberly, Stephen C. |
| 作者单位 | 1.Aix Marseille Univ, CNRS, BIP UMR 7281, IMM,FR 3479, 31 Chemin Joseph Aiguier, F-13402 Marseille 20, France 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Shanghai Sequen Bioinfo Studio, Shanghai 200092, Peoples R China 4.UK Ctr Ecol & Hydrol, Lancaster Environm Ctr, Lake Ecosyst Grp, Lib Ave, Lancaster LA1 4AP, England 5.Chinese Acad Sci, Ctr Plant Ecol, Key Lab Aquat Bot & Watershed Ecol, Core Bot Gardens,Wuhan Bot Garden, Wuhan 430074, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Wenmin,Han, Shijuan,Jiang, Hongsheng,et al. External alpha-carbonic anhydrase and solute carrier 4 are required for bicarbonate uptake in a freshwater angiosperm[J]. JOURNAL OF EXPERIMENTAL BOTANY,2020,71(19):6004-6014. |
| APA | Huang, Wenmin.,Han, Shijuan.,Jiang, Hongsheng.,Gu, Shuping.,Li, Wei.,...&Maberly, Stephen C..(2020).External alpha-carbonic anhydrase and solute carrier 4 are required for bicarbonate uptake in a freshwater angiosperm.JOURNAL OF EXPERIMENTAL BOTANY,71(19),6004-6014. |
| MLA | Huang, Wenmin,et al."External alpha-carbonic anhydrase and solute carrier 4 are required for bicarbonate uptake in a freshwater angiosperm".JOURNAL OF EXPERIMENTAL BOTANY 71.19(2020):6004-6014. |
入库方式： OAI收割
来源：武汉植物园
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