Plukenetia huayllabambana Fruits: Analysis of Bioactive Compounds, Antibacterial Activity and Relative Action Mechanisms
文献类型：期刊论文
| 作者 | Seukep, Armel Jackson2,3,4,5; Fan, Minxia3,4,5; Sarker, Satyajit Dey6; Kuete, Victor1; Guo, Ming-Quan3,4,5 |
| 刊名 | PLANTS-BASEL
 |
| 出版日期 | 2020-09-01 |
| 卷号 | 9期号:9页码:14 |
| 关键词 | Plukenetia huayllabambana solvent fractions antibacterial modes of action GC-MS |
| DOI | 10.3390/plants9091111 |
| 英文摘要 | Plukenetia huayllabambanais an edible plant traditionally used to cure wounds and various infections. The present work assessed, for the first time, the antibacterial efficacy of solvent fractions fromP. huayllabambanafruits. The crude methanol extract was obtained applying ultrasound-assisted extraction, followed by partitioning through successive depletion among solvents of increasing polarity to yield fractions (n-hexane, dichloromethane, ethyl acetate, and n-butanol). The minimal inhibitory concentration (MIC) was determined following antibacterial testing, using the broth microdilution technique against a panel of drug-resistant Gram-negative and Gram-positive bacteria. Possible modes of action of the most active fraction were also investigated. Gas chromatography-mass spectrometry (GC-MS) was used to identify phytocompounds that may account for the recorded activities. Methanol, n-hexane (PH-n-Hex), and ethyl acetate extracts inhibited 100% of studied bacteria, with the recorded MIC ranging from 0.125-1 mg/mL. PH-n-Hex appeared as the most active partition, exerting a bacteriostatic effect. PH-n-Hex probably acts by interfering with bacterial biofilm formation, proton pumps, and bacterial cell membrane integrity. The GC-MS analysis of PH-n-Hex led to the identification of 11 potentially bioactive components, including fatty acids, phytosterol, and diterpene alcohol as major ones.P. huayllabambanacan be considered as a plant of pharmacological value-a source of potent anti-infective drug entities. |
| 资助项目 | Chinese Academy of Sciences (CAS) President's International Fellowship Initiative (CAS-PIFI)[2018PB0089] ; Chinese Academy of Sciences (CAS) President's International Fellowship Initiative (CAS-PIFI)[2019VBA0026] ; Major Project for Special Technology Innovation of Hubei Province[2017AHB054] |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000580880200001 |
| 出版者 | MDPI |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/15904]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Guo, Ming-Quan |
| 作者单位 | 1.Univ Dschang, Fac Sci, Dept Biochem, Unit Res Microbiol & Antimicrobial Subst,Lab Canc, POB 67, Dschang, Cameroon 2.Univ Buea, Fac Hlth Sci, Dept Biomed Sci, POB 63, Buea, Cameroon 3.Chinese Acad Sci, CAS Key Lab Plant Germplasm Enhancement & Special, Wuhan Bot Garden, Wuhan 437004, Peoples R China 4.Chinese Acad Sci, Sino Africa Joint Res Ctr, Wuhan 437004, Peoples R China 5.Chinese Acad Sci, Innovat Acad Drug Discovery & Dev, Shanghai 201203, Peoples R China 6.Liverpool John Moores Univ, Ctr Nat Prod Discovery, Sch Pharm & Biomol Sci, Liverpool L3 3AF, Merseyside, England |
| 推荐引用方式 GB/T 7714 | Seukep, Armel Jackson,Fan, Minxia,Sarker, Satyajit Dey,et al. Plukenetia huayllabambana Fruits: Analysis of Bioactive Compounds, Antibacterial Activity and Relative Action Mechanisms[J]. PLANTS-BASEL,2020,9(9):14. |
| APA | Seukep, Armel Jackson,Fan, Minxia,Sarker, Satyajit Dey,Kuete, Victor,&Guo, Ming-Quan.(2020).Plukenetia huayllabambana Fruits: Analysis of Bioactive Compounds, Antibacterial Activity and Relative Action Mechanisms.PLANTS-BASEL,9(9),14. |
| MLA | Seukep, Armel Jackson,et al."Plukenetia huayllabambana Fruits: Analysis of Bioactive Compounds, Antibacterial Activity and Relative Action Mechanisms".PLANTS-BASEL 9.9(2020):14. |
入库方式： OAI收割
来源：武汉植物园
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