Genome-wide small RNA profiling reveals tiller development in tall fescue (Festuca arundinacea Schreb)
文献类型：期刊论文
| 作者 | Hu, Tao1,4; Wang, Tao2; Li, Huiying1,4; Wassie, Misganaw1,3,4; Xu, Huawei2; Chen, Liang1,4 |
| 刊名 | BMC GENOMICS
 |
| 出版日期 | 2020-10-06 |
| 卷号 | 21期号:1页码:13 |
| 关键词 | MicroRNAs Tiller development Small RNA deep sequencing Stem-loop qRT-PCR Tall fescue |
| ISSN号 | 1471-2164 |
| DOI | 10.1186/s12864-020-07103-x |
| 英文摘要 | BackgroundTall fescue (Festuca arundinacea Schreb.) is a major cool-season forage and turfgrass species. The low tiller density and size dramatically limits its turf performance and forage yield. MicroRNAs (miRNA)-genes modules play critical roles in tiller development in plants. In this study, a genome-wide small RNA profiling was carried out in two tall fescue genotypes contrasting for tillering production ('Ch-3', high tiller production rate and 'Ch-5', low tiller production rate) and two types of tissue samples at different tillering development stage (Pre-tillering, grass before tillering; Tillering, grass after tillering). 'Ch-3', 'Ch-5', Pre-tillering, and Tillering samples were analyzed using high-throughput RNA sequencing.ResultsA total of 222 million high-quality clean reads were generated and 208 miRNAs were discovered, including 148 known miRNAs belonging to 70 families and 60 novel ones. Furthermore, 18 miRNAs were involved in tall fescue tiller development process. Among them, 14 miRNAs displayed increased abundance in both Ch-3 and Tillering plants compared with that in Ch-5 and Pre-tillering plants and were positive with tillering, while another four miRNAs were negative with tiller development. Out of the three miRNAs osa-miR156a, zma-miR528a-3p and osa-miR444b.2, the rest of 15 miRNAs were newfound and associated with tiller development in plants. Based on our previous full-length transcriptome analysis in tall fescue, 28,927 potential target genes were discovered for all identified miRNAs. Most of the 212 target genes of the 18 miRNAs were dominantly enriched into "ubiquitin-mediated proteolysis", "phagosome", "fatty acid biosynthesis", "oxidative phosphorylation", and "biosynthesis of unsaturated fatty acids" KEGG pathways. In addition, bdi-miR167e-3p targets two kinase proteins EIF2AK4 and IRAK4, and osa-miR397a targets auxin response factor 5, which may be the significant miRNA-genes controllers in tillering development.ConclusionsThis is the first genome-wide miRNA profiles analysis to identify regulators involved in tiller development in cool-season turfgrass. Tillering related 18 miRNAs and their 212 target genes provide novel information for the understanding of the molecular mechanisms of miRNA-genes mediated tiller development in cool-season turfgrass. |
| 资助项目 | National Natural Science Foundation of China[31772349] ; National Natural Science Foundation of China[31672482] ; National Natural Science Foundation of China[31401915] ; Major Science and Technology Innovation Project of Shandong Province[2019JZZY010726] ; Poverty Alleviation through Agricultural Projects from the Agricultural Office of Chinese Academy of Sciences ; Natural Science Foundation of Henan province[182300410012] |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:000578428200008 |
| 出版者 | BMC |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/15926]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Hu, Tao; Chen, Liang |
| 作者单位 | 1.Chinese Acad Sci, Innovat Acad Seed Design, Wuhan Bot Garden, CAS Key Lab Plant Germplasm Enhancement & Special, Wuhan 430074, Peoples R China 2.Henan Univ Sci & Technol, Coll Agr, Luoyang, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Chinese Acad Sci, Core Bot Gardens, Ctr Econ Bot, Wuhan 430074, Peoples R China |
| 推荐引用方式 GB/T 7714 | Hu, Tao,Wang, Tao,Li, Huiying,et al. Genome-wide small RNA profiling reveals tiller development in tall fescue (Festuca arundinacea Schreb)[J]. BMC GENOMICS,2020,21(1):13. |
| APA | Hu, Tao,Wang, Tao,Li, Huiying,Wassie, Misganaw,Xu, Huawei,&Chen, Liang.(2020).Genome-wide small RNA profiling reveals tiller development in tall fescue (Festuca arundinacea Schreb).BMC GENOMICS,21(1),13. |
| MLA | Hu, Tao,et al."Genome-wide small RNA profiling reveals tiller development in tall fescue (Festuca arundinacea Schreb)".BMC GENOMICS 21.1(2020):13. |
入库方式： OAI收割
来源：武汉植物园
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