Comprehensive analysis of AGPase genes uncovers their potential roles in starch biosynthesis in lotus seed
文献类型：期刊论文
| 作者 | Sun, Heng2; Li, Juanjuan3; Song, Heyun1,2; Yang, Dong2,4; Deng, Xianbao2,4; Liu, Juan2; Wang, Yunmeng1,2; Ma, Junyu1,2; Xiong, Yaqian1,2; Liu, Yanling2 |
| 刊名 | BMC PLANT BIOLOGY
 |
| 出版日期 | 2020-10-06 |
| 卷号 | 20期号:1页码:15 |
| 关键词 | Lotus seed Starch biosynthesis Nutritional composition AGPase gene AGPase activity |
| ISSN号 | 1471-2229 |
| DOI | 10.1186/s12870-020-02666-z |
| 英文摘要 | Background: Starch in the lotus seed contains a high proportion of amylose, which endows lotus seed a promising property in the development of hypoglycemic and low-glycemic index functional food. Currently, improving starch content is one of the major goals for seed-lotus breeding. ADP-glucose pyrophosphorylase (AGPase) plays an essential role in regulating starch biosynthesis in plants, but little is known about its characterization in lotus. Results: We describe the nutritional compositions of lotus seed among 30 varieties with starch as a major component. Comparative transcriptome analysis showed that AGPase genes were differentially expressed in two varieties (CA and JX) with significant different starch content. Seven putative AGPase genes were identified in the lotus genome (Nelumbo nucifera Gaertn.), which could be grouped into two subfamilies. Selective pressure analysis indicated that purifying selection acted as a vital force in the evolution of AGPase genes. Expression analysis revealed that lotus AGPase genes have varying expression patterns, with NnAGPL2a and NnAGPS1a as the most predominantly expressed, especially in seed and rhizome. NnAGPL2a and NnAGPS1a were co-expressed with a number of starch and sucrose metabolism pathway related genes, and their expressions were accompanied by increased AGPase activity and starch content in lotus seed. Conclusions: Seven AGPase genes were characterized in lotus, with NnAGPL2a and NnAGPS1a, as the key genes involved in starch biosynthesis in lotus seed. These results considerably extend our understanding on lotus AGPase genes and provide theoretical basis for breeding new lotus varieties with high-starch content. |
| 资助项目 | Key Research Program of Frontier Sciences CAS[QYZDB-SSW-SMC017] ; Youth Innovation Promotion Association CAS[2017390] ; National Natural Science Foundation of China[31872136] ; National Natural Science Foundation of China[31772353] |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000578284600003 |
| 出版者 | BMC |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/15949]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Yang, Mei |
| 作者单位 | 1.Univ Chinese Acad Sci, 19A Yuquanlu, Beijing 100049, Peoples R China 2.Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan Bot Garden, Wuhan 430074, Peoples R China 3.Hubei Engn Univ, Coll Life Sci & Technol, Hubei Prov Res Ctr Engn Technol Utilizat Bot Func, Hubei Key Lab Qual Control Characterist Fruits &, Xiaogan 432000, Hubei, Peoples R China 4.Chinese Acad Sci, Ctr Econ Bot, Core Bot Gardens, Wuhan 430074, Peoples R China |
| 推荐引用方式 GB/T 7714 | Sun, Heng,Li, Juanjuan,Song, Heyun,et al. Comprehensive analysis of AGPase genes uncovers their potential roles in starch biosynthesis in lotus seed[J]. BMC PLANT BIOLOGY,2020,20(1):15. |
| APA | Sun, Heng.,Li, Juanjuan.,Song, Heyun.,Yang, Dong.,Deng, Xianbao.,...&Yang, Mei.(2020).Comprehensive analysis of AGPase genes uncovers their potential roles in starch biosynthesis in lotus seed.BMC PLANT BIOLOGY,20(1),15. |
| MLA | Sun, Heng,et al."Comprehensive analysis of AGPase genes uncovers their potential roles in starch biosynthesis in lotus seed".BMC PLANT BIOLOGY 20.1(2020):15. |
入库方式： OAI收割
来源：武汉植物园
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