A candidate PpRPH gene of the D locus controlling fruit acidity in peach
文献类型：期刊论文
| 作者 | Wang, Lu1,5; Jiang, Xiaohan1,2,5; Zhao, Li5; Wang, Furong3; Liu, Yudi2,5; Zhou, Hui5; He, Huaping3; Han, Yuepeng1,4,5 |
| 刊名 | PLANT MOLECULAR BIOLOGY
 |
| 出版日期 | 2020-10-31 |
| 页码 | 12 |
| ISSN号 | 0167-4412 |
| 关键词 | Prunus persica Fruit acidity Linkage mapping Organic acids Small protein |
| DOI | 10.1007/s11103-020-01089-6 |
| 英文摘要 | Key message A candidate gene, designate PpRPH, in the D locus was identified to control fruit acidity in peach. Fruit acidity has a strong impact on organoleptic quality of fruit. Peach fruit acidity is controlled by a large-effect D locus on chromosome 5. In this study, the D locus was mapped to a 509-kb interval, with two markers, 5dC720 and 5C1019, co-segregating with the non-acid/acid trait of peach fruit. Within this interval, a candidate gene encoding a putative small protein, designated PpRPH, showed a consistency between gene expression and fruit acidity, with up- and down-regulation in non-acidic and acidic fruits, respectively. Transient ectopic expression of PpRPH in tobacco leaves caused an increase of pH by approximately 40% compared to the control transformed with empty vector. Whereas, the concentrations of citrate and malate decreased significantly by 22% and 37%, respectively, with respect to the empty vector control. All these results suggest that PpRPH is a strong candidate gene of the D locus. These findings contribute to our overall understanding of the complex mechanism underlying fruit acidity in peach as well as that in other fruit crops. |
| 资助项目 | National Key R&D Program of China[2019YFD1000200] ; National Natural Science Foundation of China[31672134] ; China Agriculture Research System[CARS-30] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences |
| 语种 | 英语 |
| 出版者 | SPRINGER |
| WOS记录号 | WOS:000583114900001 |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/15976]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Han, Yuepeng |
| 作者单位 | 1.Chinese Acad Sci, Core Bot Gardens, Ctr Econ Bot, Wuhan 430074, Peoples R China 2.Univ Chinese Acad Sci, 19A Yuquanlu, Beijing 100049, Peoples R China 3.Hubei Acad Agr Sci, Inst Fruit Tree & Tea, Wuhan 430209, Peoples R China 4.Chinese Acad Sci, Sino African Joint Res Ctr, Wuhan, Peoples R China 5.Chinese Acad Sci, CAS Key Lab Plant Germplasm Enhancement Specialty, Wuhan Bot Garden, Innovat Acad Seed Design, Wuhan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Lu,Jiang, Xiaohan,Zhao, Li,et al. A candidate PpRPH gene of the D locus controlling fruit acidity in peach[J]. PLANT MOLECULAR BIOLOGY,2020:12. |
| APA | Wang, Lu.,Jiang, Xiaohan.,Zhao, Li.,Wang, Furong.,Liu, Yudi.,...&Han, Yuepeng.(2020).A candidate PpRPH gene of the D locus controlling fruit acidity in peach.PLANT MOLECULAR BIOLOGY,12. |
| MLA | Wang, Lu,et al."A candidate PpRPH gene of the D locus controlling fruit acidity in peach".PLANT MOLECULAR BIOLOGY (2020):12. |
入库方式： OAI收割
来源：武汉植物园
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