黑果枸杞原花青素合成相关无色花色素还原酶基因和花青素还原酶基因的克隆及表达分析
文献类型：期刊论文
| 作者 | Shen Xiaofei5; Zeng Shaohua4; Wu Min4; Liu Chunzhao3; Wang Ying5 |
| 刊名 | Journal of Chinese Pharmaceutical Sciences
 |
| 出版日期 | 2014 |
| 卷号 | 23期号:6页码:369 |
| 关键词 | 花青素还原酶 无色花色素还原酶 黑果枸杞 原花青素 |
| ISSN号 | 1003-1057 |
| 英文摘要 | Proanthocyanidins (PAs) are a set of important phytochemical compounds in Lycium ruthenicum Murr. However, PA biosynthesis in L. ruthenicum remains unclear. In this study, leucoanthocyanidin reductase (LAR) and anthocyanidin reductases (ANR) involved in PA biosynthesis were retrieved from L. ruthenicum EST database. Polymerase chain reaction (PCR) results confirmed that LrLAR and LrANR encoded a protein composed of 333 and 338 amino acids, respectively. Phylogenetic analysis showed that LrLAR and LrANR were clustered with known LAR and ANR proteins of other plants, respectively. Quantitative reversetranscription PCR (qRT-PCR) results indicated that both genes expressed in a sharp increase manner from unripe stage to color break stage and were downregulated increasingly hereafter, and that both expression levels are extremely low in young leaves, mature leaves, stems, and roots compared with fruits. Phytochemical assay revealed that the content of total PA in fruit was higher than that in young leaves, mature leaves, stems, and roots, and that the total PA level was increased sharply and then relatively stable hereafter during fruit ripening. Our results lay a foundation for uncovering the PA biosynthesis and further engineering manipulation in L. ruthenicum. |
| 语种 | 英语 |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/18058]  |
| 专题 | 中国科学院武汉植物园 |
| 作者单位 | 1.Wuhan Botanical Garden, Chinese Academy of Sciences 2.Institute of Process Engineering, Chinese Academy of Sciences 3.South China Botanical Garden, Chinese Academy of Sciences 4.South China Botanical Garden, Chinese Academy of Sciences 5.Wuhan Botanical Garden, Chinese Academy of Sciences |
| 推荐引用方式 GB/T 7714 | Shen Xiaofei,Zeng Shaohua,Wu Min,等. 黑果枸杞原花青素合成相关无色花色素还原酶基因和花青素还原酶基因的克隆及表达分析[J]. Journal of Chinese Pharmaceutical Sciences,2014,23(6):369. |
| APA | Shen Xiaofei,Zeng Shaohua,Wu Min,Liu Chunzhao,&Wang Ying.(2014).黑果枸杞原花青素合成相关无色花色素还原酶基因和花青素还原酶基因的克隆及表达分析.Journal of Chinese Pharmaceutical Sciences,23(6),369. |
| MLA | Shen Xiaofei,et al."黑果枸杞原花青素合成相关无色花色素还原酶基因和花青素还原酶基因的克隆及表达分析".Journal of Chinese Pharmaceutical Sciences 23.6(2014):369. |
入库方式： OAI收割
来源：武汉植物园
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