Isolation and genomic characterization of a cypovirus from the oleander hawk moth, Daphnis nerii
文献类型：期刊论文
| 作者 | Zhan, Zhigao1; Guan, Limei1; Wang, Jinchang1; Liu, Zhuorong1; Guo, Yan1; Xiao, Yuzhou2; Wang, Hongxiu3; Jin, Liang1 |
| 刊名 | JOURNAL OF INVERTEBRATE PATHOLOGY
 |
| 出版日期 | 2019-05-01 |
| 卷号 | 163页码:43-47 |
| 关键词 | Daphnis nerii Cypovirus Genomic characterization Full-length amplification of cDNA |
| ISSN号 | 0022-2011 |
| DOI | 10.1016/j.jip.2019.03.002 |
| 英文摘要 | The oleander hawk moth, Daphnis nerii, is a serious pest of plants belonging to the family Apocynaceae. Thus far, pathogen infection has not been reported in D. nerii. In this study, a new cytoplasmic polyhedrosis virus (cypovirus; CPV) was isolated from naturally diseased D. nerii larvae and named DnCPV-23. Virions were observed in ultrathin sections of DnCPV polyhedral bodies. Electrophoretic analysis revealed that the DnCPV genome consisted of 10 segments of double-stranded RNA (dsRNA). cDNA copies of these dsRNA segments were amplified using the method of full-length amplification of cDNAs (FLAC), cloned, and sequenced. Sequencing results showed that all segments contained one open reading frame (ORF); They shared the conserved terminal sequences AGUCAAA and AGC at 5' and 3' ends respectively, except segment 4, which is different from previously reported 22 cypoviruses. Phylogenetic analysis based on amino acid sequences of polyhedrin (encoded by segment 10) indicated that this CPV was closely related to CPV type 19. Altogether, DnCPV-23 is a new type of cypovirus. |
| 资助项目 | Key Program of Jiangxi Academy of Sciences[2017-YZD1-05] ; Biopesticide Engineering Center Program of Jiangxi Academy of Sciences[2016-YGCZX-03] ; Key Research and Development program of Jiangxi Province[20171BBF60063] ; Doctoral Program of Jiangxi Academy of Sciences[2016-XTPH1-04] ; Doctoral Program of Jiangxi Academy of Sciences[2016-YYB-04] |
| WOS研究方向 | Zoology |
| 语种 | 英语 |
| WOS记录号 | WOS:000465645400006 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/7226]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Jin, Liang |
| 作者单位 | 1.Jiangxi Acad Sci, Inst Microbiol, Nanchang 330029, Jiangxi, Peoples R China 2.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Agr & Environm Microbiol, Wuhan, Hubei, Peoples R China 3.Jiangxi Agr Acad Sci, Inst Agr Appl Microbiol, Nanchang 330200, Jiangxi, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhan, Zhigao,Guan, Limei,Wang, Jinchang,et al. Isolation and genomic characterization of a cypovirus from the oleander hawk moth, Daphnis nerii[J]. JOURNAL OF INVERTEBRATE PATHOLOGY,2019,163:43-47. |
| APA | Zhan, Zhigao.,Guan, Limei.,Wang, Jinchang.,Liu, Zhuorong.,Guo, Yan.,...&Jin, Liang.(2019).Isolation and genomic characterization of a cypovirus from the oleander hawk moth, Daphnis nerii.JOURNAL OF INVERTEBRATE PATHOLOGY,163,43-47. |
| MLA | Zhan, Zhigao,et al."Isolation and genomic characterization of a cypovirus from the oleander hawk moth, Daphnis nerii".JOURNAL OF INVERTEBRATE PATHOLOGY 163(2019):43-47. |
入库方式： OAI收割
来源：武汉植物园
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