Comparative Transcriptome Combined with Proteome Analyses Revealed Key Factors Involved in Alfalfa (Medicago sativa) Response to Waterlogging Stress
文献类型：期刊论文
| 作者 | Zeng, Ningbo1; Yang, Zhijian1; Zhang, Zhifei1; Hu, Longxing1; Chen, Liang2 |
| 刊名 | INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
 |
| 出版日期 | 2019-03-18 |
| 卷号 | 20期号:6页码:24 |
| 关键词 | alfalfa waterlogging transcriptome proteome molecular mechanism |
| ISSN号 | 1422-0067 |
| DOI | 10.3390/ijms20061359 |
| 英文摘要 | Alfalfa (Medicago sativa) is the most widely grown and most important forage crop in the world. However, alfalfa is susceptible to waterlogging stress, which is the major constraint for its cultivation area and crop production. So far, the molecular mechanism of alfalfa response to the waterlogging is largely unknown. Here, comparative transcriptome combined with proteomic analyses of two cultivars (M12, tolerant; M25, sensitive) of alfalfa showing contrasting tolerance to waterlogging were performed to understand the mechanism of alfalfa in response to waterlogging stress. Totally, 748 (581 up- and 167 down-regulated) genes were differentially expressed in leaves of waterlogging-stressed alfalfa compared with the control (M12_W vs. M12_CK), whereas 1193 (740 up- and 453 down-regulated) differentially abundant transcripts (DATs) were detected in the leaves of waterlogging-stressed plants in comparison with the control plants (M25_W vs. M25_CK). Furthermore, a total of 187 (122 up- and 65 down-regulated) and 190 (105 up- and 85 down-regulated) differentially abundant proteins (DAPs) were identified via isobaric tags for relative and absolute quantification (iTRAQ) method in M12_W vs. M12_CK and M25_W vs. M25_CK comparison, respectively. Compared dataset analysis of proteomics and transcriptomics revealed that 27 and eight genes displayed jointly up-regulated or down-regulated expression profiles at both mRNA and protein levels in M12_W vs. M12_CK comparison, whereas 30 and 27 genes were found to be co-up-regulated or co-down-regulated in M25_W vs. M25_CK comparison, respectively. The strongly enriched Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways for co-up-regulated genes at mRNA and protein levels in M12_W vs. M12_CK comparison were 'Amino sugar and nucleotide sugar metabolism', 'Arginine and proline metabolism' and 'Starch and sucrose metabolism', whereas co-up-regulated protein-related pathways including 'Arginine and proline metabolism' and 'Valine, leucine and isoleucine degradation' were largely enriched in M25_W vs. M25_CK comparison. Importantly, the identified genes related to beta-amylase, Ethylene response Factor (ERF), Calcineurin B-like (CBL) interacting protein kinases (CIPKs), Glutathione peroxidase (GPX), and Glutathione-S-transferase (GST) may play key roles in conferring alfalfa tolerance to waterlogging stress. The present study may contribute to our understanding the molecular mechanism underlying the responses of alfalfa to waterlogging stress, and also provide important clues for further study and in-depth characterization of waterlogging-resistance breeding candidate genes in alfalfa. |
| 资助项目 | Hunan Provincial Key Laboratory of Crop Germplasm Innovation and Utilization[15KFXM12] ; Science Foundation for The Excellent Youth of Hunan Provincial Education Department[17B120] ; Hunan Provincial Natural Science Foundation of China[2019JJ40135] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000464322800012 |
| 出版者 | MDPI |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/7207]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Hu, Longxing; Chen, Liang |
| 作者单位 | 1.Hunan Agr Univ, Coll Agr, Dept Pratacultural Sci, Changsha 410128, Hunan, Peoples R China 2.Chinese Acad Sci, Wuhan Bot Garden, CAS Key Lab Plant Germplasm Enhancement & Special, Wuhan 430074, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zeng, Ningbo,Yang, Zhijian,Zhang, Zhifei,et al. Comparative Transcriptome Combined with Proteome Analyses Revealed Key Factors Involved in Alfalfa (Medicago sativa) Response to Waterlogging Stress[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,2019,20(6):24. |
| APA | Zeng, Ningbo,Yang, Zhijian,Zhang, Zhifei,Hu, Longxing,&Chen, Liang.(2019).Comparative Transcriptome Combined with Proteome Analyses Revealed Key Factors Involved in Alfalfa (Medicago sativa) Response to Waterlogging Stress.INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES,20(6),24. |
| MLA | Zeng, Ningbo,et al."Comparative Transcriptome Combined with Proteome Analyses Revealed Key Factors Involved in Alfalfa (Medicago sativa) Response to Waterlogging Stress".INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 20.6(2019):24. |
入库方式： OAI收割
来源：武汉植物园
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。