The Edwardsiella piscicida Type III Translocon Protein EseC Inhibits Biofilm Formation by Sequestering EseE
文献类型：期刊论文
| 作者 | Liu, Ying Li1,2,3; He, Tian Tian1,2,3; Liu, Lu Yi1,2; Yi, Jia1,2,3; Nie, Pin1,2,4,5; Yu, Hong Bing6,7; Xie, Hai Xia1,2 |
| 刊名 | APPLIED AND ENVIRONMENTAL MICROBIOLOGY
 |
| 出版日期 | 2019-04-01 |
| 卷号 | 85期号:8页码:11 |
| 关键词 | Edwardsiella piscicida biofilm translocon protein type III secretion system |
| ISSN号 | 0099-2240 |
| DOI | 10.1128/AEM.02133-18 |
| 英文摘要 | The type III secretion system (T3SS) is one of the most important virulence factors of the fish pathogen Edwardsiella piscicida. It contains three translocon proteins, EseB, EseC, and EseD, required for translocation of effector proteins into host cells. We have previously shown that EseB forms filamentous appendages on the surface of E. piscicida, and these filamentous structures mediate bacterial cell-cell interactions promoting autoaggregation and biofilm formation. In the present study, we show that EseC, but not EseD, inhibits the autoaggregation and biofilm formation of E. piscicida. At 18 h postsubculture, a Delta eseC strain developed strong autoaggregation and mature biofilm formation, accompanied by enhanced formation of EseB filamentous appendages. This is in contrast to the weak autoaggregation and immature biofilm formation seen in the E. piscicida wild-type strain. EseE, a protein that directly binds to EseC and also positively regulates the transcription of the escC-eseE operon, was liberated and showed increased levels in the absence of EseC. This led to augmented transcription of the escC-eseE operon, thereby increasing the steady-state protein levels of intracellular EseB, EseD, and EseE, as well as biofilm formation. Notably, the levels of intracellular EseB and EseD produced by the Delta eseE and Delta eseC Delta eseE strains were similar but remarkably lower than those produced by the wild-type strain at 18 h postsubculture. Taken together, we have shown that the translocon protein EseC inhibits biofilm formation through sequestering EseE, a positive regulator of the escC-eseE operon. IMPORTANCE Edwardsiella piscicida, previously known as Edwardsiella tarda, is a Gram-negative intracellular pathogen that mainly infects fish. The type III secretion system (T3SS) plays a pivotal role in its pathogenesis. The T3SS translocon protein EseB is required for the assembly of filamentous appendages on the surface of E. piscicida. The interactions between the appendages facilitate autoaggregation and biofilm formation. In this study, we explored the role of the other two translocon proteins, EseC and EseD, in biofilm formation. We have demonstrated that EseC, but not EseD, inhibits the autoaggregation and biofilm formation of E. piscicida, providing new insights into the regulatory mechanism involved in E. piscicida biofilm formation. |
| 资助项目 | National Natural Science Foundation of China (NSFC)[31572659] ; China Agriculture Research System[CARS-46] ; Project of State Key Laboratory of Freshwater Ecology and Biotechnology[2016FBZ04] |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000463407200006 |
| 出版者 | AMER SOC MICROBIOLOGY |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/7167]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Xie, Hai Xia |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Freshwater Ecol & Biotechnol, Minist Agr, Inst Hydrobiol, Wuhan, Hubei, Peoples R China 2.Chinese Acad Sci, Inst Hydrobiol, Minist Agr, Key Lab Aquaculture Dis Control, Wuhan, Hubei, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China 5.Qingdao Agr Univ, Coll Marine Sci & Engn, Qingdao, Shandong, Peoples R China 6.Univ British Columbia, BC Childrens Hosp Res Inst, Dept Pediat, Vancouver, BC, Canada 7.Univ British Columbia, Vancouver, BC, Canada |
| 推荐引用方式 GB/T 7714 | Liu, Ying Li,He, Tian Tian,Liu, Lu Yi,et al. The Edwardsiella piscicida Type III Translocon Protein EseC Inhibits Biofilm Formation by Sequestering EseE[J]. APPLIED AND ENVIRONMENTAL MICROBIOLOGY,2019,85(8):11. |
| APA | Liu, Ying Li.,He, Tian Tian.,Liu, Lu Yi.,Yi, Jia.,Nie, Pin.,...&Xie, Hai Xia.(2019).The Edwardsiella piscicida Type III Translocon Protein EseC Inhibits Biofilm Formation by Sequestering EseE.APPLIED AND ENVIRONMENTAL MICROBIOLOGY,85(8),11. |
| MLA | Liu, Ying Li,et al."The Edwardsiella piscicida Type III Translocon Protein EseC Inhibits Biofilm Formation by Sequestering EseE".APPLIED AND ENVIRONMENTAL MICROBIOLOGY 85.8(2019):11. |
入库方式： OAI收割
来源：武汉植物园
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