Genetic and biochemical characterizations of Zika virus NS2A protein
文献类型：期刊论文
| 作者 | Zhang, Xianwen1,2,3; Xie, Xuping3; Zou, Jing3; Xia, Hongjie3; Shan, Chao3; Chen, Xinwen1,2,4; Shi, Pei-Yong3,5,6,7,8 |
| 刊名 | EMERGING MICROBES & INFECTIONS
 |
| 出版日期 | 2019-04-08 |
| 卷号 | 8期号:1页码:585-602 |
| 关键词 | Zika virus flavivirus replication virion assembly flavivirus NS2A membrane topology |
| ISSN号 | 2222-1751 |
| DOI | 10.1080/22221751.2019.1598291 |
| 英文摘要 | Zika virus (ZIKV) can cause devastating congenital Zika syndromes in pregnant women and Guillain-Barre syndrome in adults. Understanding the molecular mechanism of ZIKV replication is essential for antiviral and vaccine development. Here we report the structural and functional characterization of ZIKV NS2A protein. Biochemical structural probing suggests that ZIKV NS2A has a single segment that traverses the ER membrane and six segments that peripherally associate with the ER membrane. Functional analysis has defined distinct NS2A residues essential for viral RNA synthesis or virion assembly. Only the virion assembly-defective mutants, but not the RNA synthesis-defective mutants, could be rescued through trans complementation with a wide-type NS2A protein. These results suggest that the NS2A molecules in virion assembly complex could be recruited in trans, whereas the NS2A molecules in viral replication complex must be recruited in cis. Together with previous results, we propose a flavivirus assembly model where NS2A plays a central role in modulating viral structural and nonstructural proteins as well as genomic RNA during virion assembly. |
| 资助项目 | Centers for Disease Control and Prevention[U01CK000512] ; National Institutes of Health[AI127744] ; National Institutes of Health[AI136126] ; University of Texas Medical Branch[UL1TR-001439] ; John S. Dunn Foundation ; Amon G. Carter Foundation ; Summerfield Robert Foundation ; Robert J. Kleberg, Jr. and Helen C. Kleberg Foundation |
| WOS研究方向 | Immunology ; Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000463818100002 |
| 出版者 | TAYLOR & FRANCIS LTD |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/7107]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Xie, Xuping; Chen, Xinwen; Shi, Pei-Yong |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Univ Texas Med Branch, Dept Biochem & Mol Biol, Galveston, TX 77555 USA 4.Chinese Acad Sci, Guangzhou Inst Biomed & Hlth, Guangzhou, Guangdong, Peoples R China 5.Univ Texas Med Branch, Inst Human Infect & Immun, Galveston, TX 77555 USA 6.Univ Texas Med Branch, Sealy Inst Vaccine Sci, Galveston, TX 77555 USA 7.Univ Texas Med Branch, Sealy Ctr Struct Biol & Mol Biophys, Galveston, TX 77555 USA 8.Univ Texas Med Branch, Dept Pharmacol & Toxicol, Galveston, TX 77555 USA |
| 推荐引用方式 GB/T 7714 | Zhang, Xianwen,Xie, Xuping,Zou, Jing,et al. Genetic and biochemical characterizations of Zika virus NS2A protein[J]. EMERGING MICROBES & INFECTIONS,2019,8(1):585-602. |
| APA | Zhang, Xianwen.,Xie, Xuping.,Zou, Jing.,Xia, Hongjie.,Shan, Chao.,...&Shi, Pei-Yong.(2019).Genetic and biochemical characterizations of Zika virus NS2A protein.EMERGING MICROBES & INFECTIONS,8(1),585-602. |
| MLA | Zhang, Xianwen,et al."Genetic and biochemical characterizations of Zika virus NS2A protein".EMERGING MICROBES & INFECTIONS 8.1(2019):585-602. |
入库方式： OAI收割
来源：武汉植物园
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