Rapid direct drug susceptibility testing of Mycobacterium tuberculosis based on culture droplet digital polymerase chain reaction
文献类型：期刊论文
| 作者 | Luo, J.1,2; Luo, M.3; Li, J.1; Yu, J.1; Yang, H.1; Yi, X.3; Chen, Y.3; Wei, H.1 |
| 刊名 | INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE
 |
| 出版日期 | 2019-02-01 |
| 卷号 | 23期号:2页码:219-+ |
| 关键词 | droplet digital PCR TB drug susceptibility testing rapid test |
| ISSN号 | 1027-3719 |
| DOI | 10.5588/ijtld.18.0182 |
| 英文摘要 | SETTING: Early diagnosis and drug susceptibility testing are important for anti-tuberculosis treatment. OBJECTIVE: To develop a rapid method for detecting Mycobacterium tuberculosis and drug susceptibility based on culture and droplet digital polymerase chain reaction (ddPCR). DESIGN: M. tuberculosis in 102 sputum samples was detected using ddPCR, Xpert, quantitative PCR (qPCR) and MGIT (TM) 960 (TM). The susceptibility of ddPCR-positive samples to rifampicin (RMP), isoniazid (INH) and streptomycin (SM) was tested by measuring changes in DNA quantity over 4 days of culture. For comparison, susceptibility of MGIT 960-positive samples was tested using the standard agar proportion method. RESULTS: The sensitivity and specificity of M. tuberculosis detection using ddPCR and MGIT 960 were respectively 95.7% (95% CI 80.0-99.2) and 88.9% (95% CI 76.7-95.4). Compared with agar proportion, the susceptibility of 44 specimens positive on culture-CddPCR showed sensitivity and specificity for RMP, INH and SM of respectively 83.3% (95% CI 50.9-97.1) and 90.6% (95% CI 73.8-97.6); 79.0% (95% CI 53.9-93.0) and 92% (95% CI 72.5-98.6); 94.1% (95% CI 69.2-99.7) and 92.6% (95% CI 74.3-98.7). CONCLUSION: Culture ddPCR could detect M. tuberculosis within 5 h and drug susceptibility within 4 days directly from sputum, which would greatly reduce the laboratory time needed for tuberculosis diagnosis. |
| WOS研究方向 | Infectious Diseases ; Respiratory System |
| 语种 | 英语 |
| WOS记录号 | WOS:000459791400016 |
| 出版者 | INT UNION AGAINST TUBERCULOSIS LUNG DISEASE (I U A T L D) |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/7019]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Chen, Y.; Wei, H. |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, Key Lab Special Pathogens & Biosafety, Wuhan, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chongqing Publ Hlth Med Ctr, Chongqing 400036, Peoples R China |
| 推荐引用方式 GB/T 7714 | Luo, J.,Luo, M.,Li, J.,et al. Rapid direct drug susceptibility testing of Mycobacterium tuberculosis based on culture droplet digital polymerase chain reaction[J]. INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE,2019,23(2):219-+. |
| APA | Luo, J..,Luo, M..,Li, J..,Yu, J..,Yang, H..,...&Wei, H..(2019).Rapid direct drug susceptibility testing of Mycobacterium tuberculosis based on culture droplet digital polymerase chain reaction.INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE,23(2),219-+. |
| MLA | Luo, J.,et al."Rapid direct drug susceptibility testing of Mycobacterium tuberculosis based on culture droplet digital polymerase chain reaction".INTERNATIONAL JOURNAL OF TUBERCULOSIS AND LUNG DISEASE 23.2(2019):219-+. |
入库方式： OAI收割
来源：武汉植物园
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