Improving lupeol production in yeast by recruiting pathway genes from different organisms
文献类型：期刊论文
| 作者 | Qiao, Weibo1,2; Zhou, Zilin1,2; Liang, Qin1,2; Mosongo, Isidore1,2; Li, Changfu1,3; Zhang, Yansheng1,3 |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2019-02-28 |
| 卷号 | 9页码:8 |
| ISSN号 | 2045-2322 |
| DOI | 10.1038/s41598-019-39497-4 |
| 英文摘要 | Lupeol is a pentacyclic triterpene that shows a variety of pharmacological properties. Compared to engineering the production of sesquiterpenes and diterpenes, it is much more challenging to engineer the biosynthesis of triterpenes in microbial platforms. This study showed our efforts on engineering the triterpene pathway in Escherichia coli and Saccharomyces cerevisiae cells by recruiting the codonoptimized three lupeol pathway genes from different organisms. By comparing their activities with their respective counterparts, the squalene synthase from Thermosynechococcus elongates (tSQS), the squalene epoxidase from Rattus norvegicus (rSE) and the lupeol synthase from Olea europaea (OeLUP) were introduced into E. coli BL21(DE3), a break-through from zero was observed for lupeol biosynthesis in a prokaryotic host. We also assessed the lupeol pathway under two different yeast backgrounds-WAT11 and EPY300, and have found that the engineered strains based on EPY300, named ECHHOe, processed the best lupeol-producing ability with the maximum lupeol titer being 200.1 mg l(-1) at 30 degrees C in a 72 h-flask culture, which so far was the highest amount of lupeol obtained by a microbial system and provides a basis for further industrial application of lupeol in the future. |
| 资助项目 | National Key R&D Program of China[2018YFC1706200] ; National Science and Technology Program of China[2012AA02A704] ; Hubei Renyue Pharmaceutical Co. LTD[492014-026053] |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000459891700003 |
| 出版者 | NATURE PUBLISHING GROUP |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/7017]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Zhang, Yansheng |
| 作者单位 | 1.Chinese Acad Sci, CAS Key Lab Plant Germplasm Enhancement & Special, Wuhan Bot Garden, Wuhan 430074, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Shanghai Univ, Res Ctr Nat Prod, Sch Life Sci, Shanghai Key Lab Bioenergy Crops, Shanghai 200444, Peoples R China |
| 推荐引用方式 GB/T 7714 | Qiao, Weibo,Zhou, Zilin,Liang, Qin,et al. Improving lupeol production in yeast by recruiting pathway genes from different organisms[J]. SCIENTIFIC REPORTS,2019,9:8. |
| APA | Qiao, Weibo,Zhou, Zilin,Liang, Qin,Mosongo, Isidore,Li, Changfu,&Zhang, Yansheng.(2019).Improving lupeol production in yeast by recruiting pathway genes from different organisms.SCIENTIFIC REPORTS,9,8. |
| MLA | Qiao, Weibo,et al."Improving lupeol production in yeast by recruiting pathway genes from different organisms".SCIENTIFIC REPORTS 9(2019):8. |
入库方式： OAI收割
来源：武汉植物园
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