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Different responses in one-year-old and three-year-old grass carp reveal the mechanism of age restriction of GCRV infection

文献类型：期刊论文

作者Chen, Geng1,2; Xiong, Lv1,2; Wang, Yumeng1,3; He, Libo1; Huang, Rong1; Liao, Lanjie1; Zhu, Zuoyan1; Wang, Yaping1
刊名FISH & SHELLFISH IMMUNOLOGY
出版日期2019-03-01
卷号86页码:702-712
关键词Grass carp Grass carp reovirus Age restriction Complement DNA methylation
ISSN号1050-4648
DOI10.1016/j.fsi.2018.11.074
英文摘要Grass carp is an important fish species in Chinese aquaculture, and can be afflicted by a hemorrhagic disease caused by the grass carp reovirus (GCRV). Interestingly, the affects of GCRV infection of grass carp are age-restricted, meaning that one-year-old grass carp can be infected and can suffer hemorrhagic disease, but three-year-old carp are not so afflicted. In this study, we investigated the mechanism responsible for this age-restricted pathology. We evaluated the relative copy number of GCRV RNA, the expression levels of proteins in blood, and changes in DNA methylation in carp from the two age groups after infection with GCRV. After GCRV infection, the relative copy number of GCRV RNA in three-year-old grass carp was significantly lower than in one-year-old carp. The differences in circulating protein levels mainly occurred in concentrated in complement and coagulation proteins, and the expression levels of these proteins were significantly higher in three-year-old grass carp than in one-year-old carp. Moreover, the expression levels of DNA methylation-related genes in the liver and spleen of one-year-old grass carp were significantly higher than those of three-year-old carp. These results suggested that as age of grass carp increases, faster and more efficient response of the immune system after viral infection, especially the complement system, and differences in DNA methylation may be important factors that affect the age restriction observed in GCRV infection. Our study provides new insights into the mechanisms underlying age restriction of GCRV infection.
资助项目National Natural Science Foundation of China[31721005] ; National Natural Science Foundation of China[31702332]
WOS研究方向Fisheries ; Immunology ; Marine & Freshwater Biology ; Veterinary Sciences
语种英语
WOS记录号WOS:000459528400079
出版者ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
源URL[http://202.127.146.157/handle/2RYDP1HH/6856]  
专题中国科学院武汉植物园
通讯作者Wang, Yaping
作者单位1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei, Peoples R China
2.Univ Chinese Acad Sci, Beijing, Peoples R China
3.Wuhan Univ, Coll Life Sci, Wuhan, Hubei, Peoples R China
推荐引用方式
GB/T 7714		 Chen, Geng,Xiong, Lv,Wang, Yumeng,et al. Different responses in one-year-old and three-year-old grass carp reveal the mechanism of age restriction of GCRV infection[J]. FISH & SHELLFISH IMMUNOLOGY,2019,86:702-712.
APA Chen, Geng.,Xiong, Lv.,Wang, Yumeng.,He, Libo.,Huang, Rong.,...&Wang, Yaping.(2019).Different responses in one-year-old and three-year-old grass carp reveal the mechanism of age restriction of GCRV infection.FISH & SHELLFISH IMMUNOLOGY,86,702-712.
MLA Chen, Geng,et al."Different responses in one-year-old and three-year-old grass carp reveal the mechanism of age restriction of GCRV infection".FISH & SHELLFISH IMMUNOLOGY 86(2019):702-712.

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来源：武汉植物园

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