A Ma10 gene encoding P-type ATPase is involved in fruit organic acid accumulation in apple
文献类型：期刊论文
| 作者 | Ma, Baiquan1,2; Liao, Liao1,3; Fang, Ting1,4; Peng, Qian1,4; Ogutu, Collins1,4; Zhou, Hui1,3; Ma, Fengwang2; Han, Yuepeng1,3 |
| 刊名 | PLANT BIOTECHNOLOGY JOURNAL
 |
| 出版日期 | 2019-03-01 |
| 卷号 | 17期号:3页码:674-686 |
| 关键词 | Malus domestica fruit acidity P-type ATPase transcriptome analysis candidate gene association mapping |
| ISSN号 | 1467-7644 |
| DOI | 10.1111/pbi.13007 |
| 英文摘要 | Acidity is one of the main determinants of fruit organoleptic quality. Here, comparative transcriptome analysis was conducted between two cultivars that showed a significant difference in fruit acidity, but contained homozygous non-functional alleles at the major gene Ma1 locus controlling apple fruit acidity. A candidate gene for fruit acidity, designated M10, was identified. The M10 gene encodes a P-type proton pump, P-3A-ATPase, which facilitates malate uptake into the vacuole. The Ma10 gene is significantly associated with fruit malate content, accounting for similar to 7.5% of the observed phenotypic variation in apple germplasm. Subcellular localization assay showed that the Ma10 is targeted to the tonoplast. Overexpression of the Ma10 gene can complement the defect in proton transport of the mutant YAK2 yeast strain and enhance the accumulation of malic acid in apple callus. Moreover, its ectopic expression in tomato induces a decrease in fruit pH. These results suggest that the Ma10 gene has the capacity for proton pumping and plays an important role in fruit vacuolar acidification in apple. Our study provides useful knowledge towards comprehensive understanding of the complex mechanism regulating apple fruit acidity. |
| 资助项目 | National Natural Science Foundation of China[31420103914] ; National Natural Science Foundation of China[31372048] ; Overseas Construction Plan for Science and Education Base, China-Africa Center for Research and Education, Chinese Academy of Sciences[SAJC201327] |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000459220300011 |
| 出版者 | WILEY |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/6843]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Han, Yuepeng |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan, Hubei, Peoples R China 2.Northwest A&F Univ, Coll Hort, State Key Lab Crop Stress Biol Arid Areas, Shaanxi Key Lab Apple, Yangling, Shaanxi, Peoples R China 3.Chinese Acad Sci, Sino African Joint Res Ctr, Wuhan, Hubei, Peoples R China 4.Chinese Acad Sci, Grad Univ, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ma, Baiquan,Liao, Liao,Fang, Ting,et al. A Ma10 gene encoding P-type ATPase is involved in fruit organic acid accumulation in apple[J]. PLANT BIOTECHNOLOGY JOURNAL,2019,17(3):674-686. |
| APA | Ma, Baiquan.,Liao, Liao.,Fang, Ting.,Peng, Qian.,Ogutu, Collins.,...&Han, Yuepeng.(2019).A Ma10 gene encoding P-type ATPase is involved in fruit organic acid accumulation in apple.PLANT BIOTECHNOLOGY JOURNAL,17(3),674-686. |
| MLA | Ma, Baiquan,et al."A Ma10 gene encoding P-type ATPase is involved in fruit organic acid accumulation in apple".PLANT BIOTECHNOLOGY JOURNAL 17.3(2019):674-686. |
入库方式： OAI收割
来源：武汉植物园
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