Herpes Simplex Virus Type 2 Immediate Early Protein ICP27 Inhibits IFN-beta Production in Mucosal Epithelial Cells by Antagonizing IRF3 Activation
文献类型：期刊论文
| 作者 | Guan, Xinmeng1,2; Zhang, Mudan3; Fu, Ming1,2; Luo, Sukun4; Hu, Qinxue1,5 |
| 刊名 | FRONTIERS IN IMMUNOLOGY
 |
| 出版日期 | 2019-02-26 |
| 卷号 | 10页码:14 |
| ISSN号 | 1664-3224 |
| 关键词 | HSV-2 ICP27 epithelial cells IFN-beta IRF3 |
| DOI | 10.3389/fimmu.2019.00290 |
| 英文摘要 | Herpes simplex virus type 2 (HSV-2) is the main cause of genital herpes and infections are common in the lower genital tract. Although neuronal and immune cells can be infected, epithelial cells, and keratinocytes are the primary HSV-2 target cells. HSV-2 establishes latency by evading the host immune system and its infection can also increase the risk of HIV-1 sexual transmission. Our pervious study found that HSV-2 immediate early protein ICP22, inhibited IFN-beta production by interfering with the IRF3 pathway. However, ICP22-null HSV-2 did not completely lose the capability of suppressing IFN-beta induction, suggesting the involvement of other viral components in the process. In this study, by using an ex vivo cervical explant model, we first demonstrated that HSV-2 can indeed inhibit IFN-beta induction in human mucosal tissues. We further identified HSV-2 immediate early protein ICP27 as a potent IFN-beta antagonist. ICP27 significantly suppresses the Sendai virus or polyinosinic-polycytidylic acid-induced IFN-beta production in human mucosal epithelial cells, showing that ICP27 inhibits the IFN-beta promoter activation, and IFN-beta production at both mRNA and protein levels. Additional studies revealed that ICP27 directly associates with IRF3 and inhibits its phosphorylation and nuclear translocation, resulting in the inhibition of IFN-beta induction. Our findings provide insights into the molecular mechanism underlying HSV-2 mucosal immune evasion, and information for the design of HSV-2 mucosal vaccines. |
| 资助项目 | National Natural Science Foundation of China[81772192] ; National Natural Science Foundation of China[81572009] |
| WOS研究方向 | Immunology |
| 语种 | 英语 |
| 出版者 | FRONTIERS MEDIA SA |
| WOS记录号 | WOS:000459675000001 |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/6835]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Zhang, Mudan; Hu, Qinxue |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Inst Virol, State Key Lab Virol, Wuhan, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Wuhan Inst Virol, Guangzhou Women & Childrens Med Ctr, Joint Ctr Translat Precis Med,Guangzhou Inst Pedi, Wuhan, Hubei, Peoples R China 4.Huazhong Univ Sci & Technol, Tongji Med Coll, Wuhan Childrens Hosp, Wuhan Maternal & Child Healthcare Hosp, Wuhan, Hubei, Peoples R China 5.St Georges Univ London, Inst Infect & Immun, London, England |
| 推荐引用方式 GB/T 7714 | Guan, Xinmeng,Zhang, Mudan,Fu, Ming,et al. Herpes Simplex Virus Type 2 Immediate Early Protein ICP27 Inhibits IFN-beta Production in Mucosal Epithelial Cells by Antagonizing IRF3 Activation[J]. FRONTIERS IN IMMUNOLOGY,2019,10:14. |
| APA | Guan, Xinmeng,Zhang, Mudan,Fu, Ming,Luo, Sukun,&Hu, Qinxue.(2019).Herpes Simplex Virus Type 2 Immediate Early Protein ICP27 Inhibits IFN-beta Production in Mucosal Epithelial Cells by Antagonizing IRF3 Activation.FRONTIERS IN IMMUNOLOGY,10,14. |
| MLA | Guan, Xinmeng,et al."Herpes Simplex Virus Type 2 Immediate Early Protein ICP27 Inhibits IFN-beta Production in Mucosal Epithelial Cells by Antagonizing IRF3 Activation".FRONTIERS IN IMMUNOLOGY 10(2019):14. |
入库方式： OAI收割
来源：武汉植物园
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