Deep sequencing of small RNAs from 11 tissues of grass carp Ctenopharyngodon idella and discovery of sex-related microRNAs
文献类型：期刊论文
| 作者 | Chen, Liangming1,2; Huang, Rong1; Zhu, Denghui1,2; Yang, Cheng1,2; He, Libo1; Li, Yongming1; Liao, Lanjie1; Zhu, Zuoyan1; Wang, Yaping1 |
| 刊名 | JOURNAL OF FISH BIOLOGY
 |
| 出版日期 | 2019 |
| 卷号 | 94期号:1页码:132-141 |
| 关键词 | deep sequencing dmrt grass carp microRNA sex differentiation |
| ISSN号 | 0022-1112 |
| DOI | 10.1111/jfb.13875 |
| 英文摘要 | This research identified 169 known microRNAs (miRNAs), 380 novel miRNAs, and 30,538 targets in 11 tissues (blood, brain, derma, gill, heart, intestine, kidney, liver, muscle, pronephros, and spleen) from grass carp Ctenopharyngodon idella with high-throughput sequencing (HTS). Transcripts per million (TPM) expression analysis detected 41 brain-enriched miRNAs (accounting for 61.19% of all tissue-enriched miRNAs). Real-time quantitative PCR (RT-qPCR) confirmed that 21 of 24 randomly selected tissue-enriched miRNAs from the TPM analysis were indeed tissue-enriched (P < 0.05), suggesting the HTS and TPM analyses were reliable. Nine of the 41 brain-enriched miRNAs are complementary to members of the double-sex and mab-3 related transcription factor family (dmrt) involved in sex differentiation. RT-qPCR revealed that cid-miR-138 was more highly expressed in testis than in ovary (P < 0.01), while the reverse was true for target gene dmrt4a (P < 0.01). This opposite expression pattern suggested the direct participation of cid-miR-138-dmrt4a in neuroendocrine mechanisms related to brain-pituitary networks during sex development. The discovery of miRNAs from 11 C. idella tissues expands the available fish miRNA database, and enhances our understanding of the role of sex-related miRNAs in tissue differentiation and maintenance of specific tissue functions in fishes. |
| 资助项目 | National Natural Science Foundation of China[31572614] ; Strategic Priority Research Program of Chinese Academy of Sciences[XDA08030203] ; State of Key Laboratory of Freshwater Ecology and Biotechnology[2016FBZ02] |
| WOS研究方向 | Fisheries ; Marine & Freshwater Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000457463200015 |
| 出版者 | WILEY |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/6739]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Wang, Yaping |
| 作者单位 | 1.Chinese Acad Sci, Inst Hydrobiol, State Key Lab Freshwater Ecol & Biotechnol, Wuhan, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Liangming,Huang, Rong,Zhu, Denghui,et al. Deep sequencing of small RNAs from 11 tissues of grass carp Ctenopharyngodon idella and discovery of sex-related microRNAs[J]. JOURNAL OF FISH BIOLOGY,2019,94(1):132-141. |
| APA | Chen, Liangming.,Huang, Rong.,Zhu, Denghui.,Yang, Cheng.,He, Libo.,...&Wang, Yaping.(2019).Deep sequencing of small RNAs from 11 tissues of grass carp Ctenopharyngodon idella and discovery of sex-related microRNAs.JOURNAL OF FISH BIOLOGY,94(1),132-141. |
| MLA | Chen, Liangming,et al."Deep sequencing of small RNAs from 11 tissues of grass carp Ctenopharyngodon idella and discovery of sex-related microRNAs".JOURNAL OF FISH BIOLOGY 94.1(2019):132-141. |
入库方式： OAI收割
来源：武汉植物园
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