Sequential phototrophic-mixotrophic cultivation of oleaginous microalga Graesiella sp. WBG-1 in a 1000m(2) open raceway pond
文献类型：期刊论文
| 作者 | Wen, Xiaobin1; Tao, Huanping1; Peng, Xinan1,3; Wang, Zhongjie1; Ding, Yi1; Xu, Yan1; Liang, Lin1,2; Du, Kui1,4; Zhang, Aoqi1,2; Liu, Caixia1,2 |
| 刊名 | BIOTECHNOLOGY FOR BIOFUELS
 |
| 出版日期 | 2019-02-11 |
| 卷号 | 12页码:14 |
| 关键词 | Microalgae cultivation Graesiella Mixotrophic Open raceway pond SPMC |
| ISSN号 | 1754-6834 |
| DOI | 10.1186/s13068-019-1367-1 |
| 英文摘要 | BackgroundMicroalgae are an important feedstock in industries. Currently, efforts are being made in the non-phototrophic cultivation of microalgae for biomass production. Studies have shown that mixotrophy is a more efficient process for producing algal biomass in comparison to phototrophic and heterotrophic cultures. However, cultivation of microalgae in pilot-scale open ponds in the presence of organic carbon substrates has not yet been developed. The problems are heterotrophic bacterial contamination and inefficient conversion of organic carbon.ResultsLaboratory investigation was combined with outdoor cultivation to find a culture condition that favors the growth of alga, but inhibits bacteria. A window period for mixotrophic cultivation of the alga Graesiella sp. WBG-1 was identified. Using this period, a new sequential phototrophic-mixotrophic cultivation (SPMC) method that enhances algal biomass productivity and limits bacteria contamination at the same time was established for microalgae cultivation in open raceway ponds. Graesiella sp. WBG-1 maximally produced 12.5g biomass and 4.1g lipids m(-2)day(-1) in SPMC in a 1000m(2) raceway pond, which was an over 50% increase compared to phototrophic cultivation. The bacterial number in SPMC (2.97x10(5) CFUml(-1)) is comparable to that of the phototrophic cultivations.ConclusionsSPMC is an effective and feasible method to cultivate lipid-rich microalgae in open raceway ponds. Successful scale-up of SPMC in a commercial raceway pond (1000m(2) culture area) was demonstrated for the first time. This method is attractive for global producers of not only lipid-rich microalgae biomass, but also astaxanthin and -carotene. |
| 资助项目 | National High-tech R&D Program of China[2013AA065805] ; Science and Technology Service Network Initiative of Chinese Academy of Sciences[KFJ-SW-STS-163] |
| WOS研究方向 | Biotechnology & Applied Microbiology ; Energy & Fuels |
| 语种 | 英语 |
| WOS记录号 | WOS:000458364300001 |
| 出版者 | BMC |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/6651]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Li, Yeguang |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Bot Garden, CAS Key Lab Plant Germplasm Enhancement & Special, Wuhan 430074, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Zhengzhou Normal Univ, Inst Bioengn, Zhengzhou 450044, Henan, Peoples R China 4.Sichuan Prov Acad Nat Resource Sci, Chengdu 610015, Sichuan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wen, Xiaobin,Tao, Huanping,Peng, Xinan,et al. Sequential phototrophic-mixotrophic cultivation of oleaginous microalga Graesiella sp. WBG-1 in a 1000m(2) open raceway pond[J]. BIOTECHNOLOGY FOR BIOFUELS,2019,12:14. |
| APA | Wen, Xiaobin.,Tao, Huanping.,Peng, Xinan.,Wang, Zhongjie.,Ding, Yi.,...&Li, Yeguang.(2019).Sequential phototrophic-mixotrophic cultivation of oleaginous microalga Graesiella sp. WBG-1 in a 1000m(2) open raceway pond.BIOTECHNOLOGY FOR BIOFUELS,12,14. |
| MLA | Wen, Xiaobin,et al."Sequential phototrophic-mixotrophic cultivation of oleaginous microalga Graesiella sp. WBG-1 in a 1000m(2) open raceway pond".BIOTECHNOLOGY FOR BIOFUELS 12(2019):14. |
入库方式： OAI收割
来源：武汉植物园
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