Characterization of functional transposable element enhancers in acute myeloid leukemia
文献类型：期刊论文
| 作者 | Zeng, Yingying5; Cao, Yaqiang5; Liu, Denghui5; Zhang, Xiaoli5; Han, Jing-Dong J.5; Halevy, Rivka Sukenik2,3,4; Nguyen, Picard3,4; Ahituv, Nadav3,4; Han, Jing-Dong J.1; , |
| 刊名 | SCIENCE CHINA-LIFE SCIENCES
 |
| 出版日期 | 2020 |
| 卷号 | 63期号:5页码:675-687 |
| 关键词 | SET ENRICHMENT ANALYSIS C-JUN GENE GENOME CHROMATIN EVOLUTION MLL-AF9 PROTEIN DNA DIFFERENTIATION |
| ISSN号 | 1674-7305 |
| DOI | 10.1007/s11427-019-1574-x |
| 文献子类 | Article |
| 英文摘要 | Transposable elements (TEs) have been shown to have important gene regulatory functions and their alteration could lead to disease phenotypes. Acute myeloid leukemia (AML) develops as a consequence of a series of genetic changes in hematopoietic precursor cells, including mutations in epigenetic factors. Here, we set out to study the gene regulatory role of TEs in AML. We first explored the epigenetic landscape of TEs in AML patients using ATAC-seq data. We show that a large number of TEs in general, and more specifically mammalian-wide interspersed repeats (MIRs), are more enriched in AML cells than in normal blood cells. We obtained a similar finding when analyzing histone modification data in AML patients. Gene Ontology enrichment analysis showed that genes near MIRs in open chromatin regions are involved in leukemogenesis. To functionally validate their regulatory role, we selected 19 MIR regions in AML cells, and tested them for enhancer activity in an AML cell line (Kasumi-1) and a chronic myeloid leukemia (CML) cell line (K562); the results revealed several MIRs to be functional enhancers. Taken together, our results suggest that TEs are potentially involved in myeloid leukemogenesis and highlight these sequences as potential candidates harboring AML-associated variation. |
| 学科主题 | Biology |
| WOS关键词 | transposable element ; enhancer ; promoter ; MIR ; acute myeloid leukemia |
| 语种 | 英语 |
| WOS记录号 | WOS:000527942100003 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/3552]  |
| 专题 | 生化所2019-2020年发文 |
| 作者单位 | 1.Peking Univ, Ctr Quantitat Biol, Acad Adv Interdisciplinary Studies, Peking Tsinghua Ctr Life Sci, Beijing 100871, Peoples R China, 2.Tel Aviv Univ, Sackler Sch Med, IL-6997801 Tel Aviv, Israel; 3.Univ Calif San Francisco, Inst Human Genet, San Francisco, CA 94143 USA; 4.Univ Calif San Francisco, Dept Bioengn & Therapeut Sci, San Francisco, CA 94158 USA; 5.Chinese Acad Sci, Shanghai Inst Biol Sci,CAS Key Lab Computat, Collaborat Innovat Ctr Genet & Dev Biol,CAS MPG P, Ctr Excellence Mol Cell Sci,Shanghai Inst Nutr &, Shanghai 200031, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Zeng, Yingying,Cao, Yaqiang,Liu, Denghui,et al. Characterization of functional transposable element enhancers in acute myeloid leukemia[J]. SCIENCE CHINA-LIFE SCIENCES,2020,63(5):675-687. |
| APA | Zeng, Yingying.,Cao, Yaqiang.,Liu, Denghui.,Zhang, Xiaoli.,Han, Jing-Dong J..,...&,.(2020).Characterization of functional transposable element enhancers in acute myeloid leukemia.SCIENCE CHINA-LIFE SCIENCES,63(5),675-687. |
| MLA | Zeng, Yingying,et al."Characterization of functional transposable element enhancers in acute myeloid leukemia".SCIENCE CHINA-LIFE SCIENCES 63.5(2020):675-687. |
入库方式： OAI收割
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。