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  4. 生化所2019-2020年发文
Roles of TGF beta 1 in the expression of phosphoinositide 3-kinase isoform genes and sensitivity and response of lung telocytes to PI3K inhibitors

文献类型：期刊论文

作者Song, Dongli3; Tang, Li3; Wang, Lu3; Huang, Jianan3; Wang, Xiangdong3; Zeng, Tao2; Fang, Hao1; ,
刊名CELL BIOLOGY AND TOXICOLOGY
出版日期2020
卷号36期号:1页码:51-64
ISSN号0742-2091
关键词MESENCHYMAL STEM-CELLS AIRWAY EPITHELIAL-CELLS TGF-BETA II CELLS PULMONARY TELOCYTES CANCER CELLS FIBROBLASTS PROLIFERATION
DOI10.1007/s10565-019-09487-3
文献子类Article
英文摘要Background The mouse lung telocyte cell line (TCSV40) recently established provides further opportunities to learn TC biology and functions. The present study aims at investigating regulatory roles of phosphoinositide 3-kinase (PI3K) isoforms in TC proliferation and movement and in TGF beta 1-induced sensitivity and response of lung TCs to PI3K inhibitors. Materials and methods Network and molecular interactions of genes coding PI3K family or TGF beta family proteins in mouse primary TCs were defined. Mouse lung TCSV40 proliferation, apoptosis, cell cycle, and dynamical bio-behaviors were measured with or without TGF beta 1 stimulation or PI3K catalytic isoform protein (PI3K/mTOR, PI3K alpha/delta/beta, PI3K p110 delta, or pan-PI3K) inhibitions. Results The present study showed the difference of network characteristics and interactions of genes coding PI3K isoform proteins or TGF beta family proteins in primary lung telocytes from mouse lungs compared to those of other cells residing in the lung. TGF beta 1 had diverse effects on TC proliferation with altered TC number in G2 or S phase, independent upon the administered dose of TGF beta 1. PI3K alpha/delta/beta, PI3K/mTOR, and PI3K p110 delta were involved in TC proliferation, of which PI3K alpha/delta/beta was more sensitive. The effects of pan-PI3K inhibitor indicate that more PI3K isoforms were stimulated by the administering of external TGF beta 1 and contributed to TGF beta 1-induced TC proliferation. PI3K p110 delta upregulated TC proliferation and movement dynamically without TGF beta 1, and downregulated TC proliferation with TGF beta 1 stimulation, but not TC movement. PI3K alpha/delta/beta and PI3K/mTOR were more active in TGF beta 1-induced S phase accumulation and had similar dynamic effects to PI3K p110 delta. Gene expression of PI3K isoforms in TCs was upregulated after TGF beta 1 stimulation. The expression of PIK3CA coding p110-alpha or PIK3CG coding p110-gamma were up- or downregulated in TCs without TGF beta 1, respectively, when PI3K/mTOR, PI3K alpha/delta/beta, PI3K p110 delta, or pan-PI3K were inhibited. TGF beta 1 upregulated the expression of PIK3CA and PIK3CB, while downregulated the expression of PIK3CD and PIK3CG. Conclusion Our data imply that TGF beta 1 plays divergent roles in the expression of PI3K isoform genes in lung TCs and can alter the sensitivity and response of lung TCs to PI3K inhibitors.
学科主题Cell Biology ; Toxicology
WOS关键词Telocyte ; TGF beta 1 ; Lung ; PI3K isoforms ; Cell proliferation
语种英语
WOS记录号WOS:000519075600006
版本出版稿
源URL[http://202.127.25.143/handle/331003/3575]  
专题生化所2019-2020年发文
作者单位1.Fudan Univ, Dept Anesthesiol, Zhongshan Hosp, Minhang Branch, Shanghai, Peoples R China,
2.Chinese Acad Sci, Inst Biochem & Cell Biol, Shanghai Inst Biol Sci, Key Lab Syst Biol, Shanghai, Peoples R China;
3.Fudan Univ, Zhongshan Hosp Inst Clin Sci, Shanghai Inst Clin Bioinformat,Shanghai Med Coll, Shanghai Engn Res Technol Cardiopulm Dis,Jinshan, Shanghai, Peoples R China;
推荐引用方式
GB/T 7714		 Song, Dongli,Tang, Li,Wang, Lu,et al. Roles of TGF beta 1 in the expression of phosphoinositide 3-kinase isoform genes and sensitivity and response of lung telocytes to PI3K inhibitors[J]. CELL BIOLOGY AND TOXICOLOGY,2020,36(1):51-64.
APA Song, Dongli.,Tang, Li.,Wang, Lu.,Huang, Jianan.,Wang, Xiangdong.,...&,.(2020).Roles of TGF beta 1 in the expression of phosphoinositide 3-kinase isoform genes and sensitivity and response of lung telocytes to PI3K inhibitors.CELL BIOLOGY AND TOXICOLOGY,36(1),51-64.
MLA Song, Dongli,et al."Roles of TGF beta 1 in the expression of phosphoinositide 3-kinase isoform genes and sensitivity and response of lung telocytes to PI3K inhibitors".CELL BIOLOGY AND TOXICOLOGY 36.1(2020):51-64.

入库方式： OAI收割

来源：上海生物化学与细胞生物学研究所

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