Temporal regulation of prenatal embryonic development by paternal imprinted loci
文献类型：期刊论文
| 作者 | Li, Qing2; Li, Yuanyuan2; Yin, Qi2; Wang, Kai2; Li, Wei2; Chang, Boran2; Li, Jinsong1,2; Huang, Shuo1; Zhuo, Liangchai1; , |
| 刊名 | SCIENCE CHINA-LIFE SCIENCES
 |
| 出版日期 | 2020 |
| 卷号 | 63期号:1页码:2020-01-17 |
| 关键词 | ENHANCER-BLOCKING ACTIVITY H19 GENE IGF-II MOUSE MICE CELLS EXPRESSION GENERATION METHYLATION PROTEIN |
| ISSN号 | 1674-7305 |
| DOI | 10.1007/s11427-019-9817-6 |
| 文献子类 | Article |
| 英文摘要 | Paternal imprinted genes (H19 and Gtl2) are pivotal for prenatal embryonic development in mice. Nongrowing oocytes and sperm- or oocyte-originated haploid embryonic stem cells (haESCs) carrying both H19-DMR (differentially DNA-methylated region) and IG (intergenic)-DMR deletions that partially mimic paternal imprinting of H19-Igf2 and Dlk1-Dio3 can be employed as sperm replacement to efficiently support full-term embryonic development. However, how H19-DMR and IG-DMR act together to regulate embryonic development is still largely unknown. Here, using androgenetic haESC (AG-haESC)-mediated semi-cloned (SC) technology, we showed that paternal H19-DMR and IG-DMR are not essential for pre-implantation development of SC embryos generated through injection of AG-haESCs into oocytes. H19-DMR plays critical roles before 12.5 days of gestation while IG-DMR is essential for late-gestation of SC embryos. Interestingly, we found that combined deletions of H19 and H19-DMR can further improve the efficiency of normal development of SC embryos at mid-gestation compared to DKO SC embryos. Transcriptome and histology analyses revealed that H19 and H19-DMR combined deletions rescue the placental defects. Furthermore, we showed that H19, H19-DMR and IG-DMR deletions (TKO) give rise to better prenatal and postnatal embryonic development of SC embryos compared to DKO. Together, our results indicate the temporal regulation of paternal imprinted loci during embryonic development. |
| 学科主题 | Biology |
| WOS关键词 | imprinted loci ; semi-cloned technology ; temporal regulation ; H19-Igf2 ; Dlk1-Dio3 ; embryonic development |
| 语种 | 英语 |
| WOS记录号 | WOS:000511500100001 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/3580]  |
| 专题 | 生化所2019-2020年发文 |
| 作者单位 | 1.Shanghai Tech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China, 2.Chinese Acad Sci, Univ Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol,State Key Lab C, Shanghai Key Lab Mol Androl,CAS Ctr Excellence Mo, Shanghai 200031, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Li, Qing,Li, Yuanyuan,Yin, Qi,et al. Temporal regulation of prenatal embryonic development by paternal imprinted loci[J]. SCIENCE CHINA-LIFE SCIENCES,2020,63(1):2020-01-17. |
| APA | Li, Qing.,Li, Yuanyuan.,Yin, Qi.,Wang, Kai.,Li, Wei.,...&,.(2020).Temporal regulation of prenatal embryonic development by paternal imprinted loci.SCIENCE CHINA-LIFE SCIENCES,63(1),2020-01-17. |
| MLA | Li, Qing,et al."Temporal regulation of prenatal embryonic development by paternal imprinted loci".SCIENCE CHINA-LIFE SCIENCES 63.1(2020):2020-01-17. |
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