Integrated analyses reveal hsa_circ_0028883 as a diagnostic biomarker in active tuberculosis
文献类型：期刊论文
| 作者 | Zhang, Xiaolin5; Wu, Qingguo5; Tang, Haicheng5; Zhang, Yunbin5; Li, Feng5; Zhang, Qian4; Ye, Linxiong4; Zhang, Qilong3; Hua, Demi2; Zhang, Yunbin1 |
| 刊名 | INFECTION GENETICS AND EVOLUTION
 |
| 出版日期 | 2020 |
| 卷号 | 83页码:104323 |
| 关键词 | CIRCULAR RNA EXPRESSION MOLECULES TARGET GENES |
| ISSN号 | 1567-1348 |
| DOI | 10.1016/j.meegid.2020.104323 |
| 文献子类 | Article |
| 英文摘要 | Circular RNAs (circRNAs) are known to be closely involved in various diseases progression. Nevertheless, their function and underlying mechanisms in tuberculosis (TB) remain largely unknown. The aim of the present study was to explore their potential diagnostic values in TB. We downloaded the gene expression datasets of circRNA (GSE117563 and GSE106953), microRNA (miRNA, dataset GSE29190) and mRNA (GSE54992) from Gene Expression Omnibus (GEO) database. A competing endogenous RNAs (ceRNA) network was constructed based on circRNA-miRNA-mRNA potential interaction. We also constructed a circRNA-miRNA-hub gene regulatory module by using the Cytohubba. Gene ontology (GO) as well as Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis were used to predict their biological functions. By further validation, the expression level of hsa_circ_0028883 and hsa-miR-409-5p were detected by qRT-PCR in 20 active TB patients and 20 healthy donors. Then, Receiver Operating Characteristic (ROC) was constructed to evaluate the diagnostic values of hsa_circ_0028883. 1 differentially expressed circRNA (DE-circRNA), 1 differentially expressed miRNA (DE-miRNA), and 44 differentially expressed mRNAs (DE-mRNAs) were selected for the construction of ceRNA network in TB. A circRNA-miRNA-hub gene (mRNA) sub-network was constructed based on 1 DE-circRNA, 1 DE-miRNA, and 8 DE-mRNAs. Hsa_circ_0028883/hsa-miR-409-5p/mRNA interactions may provide some novel mechanisms for active TB. GO and KEGG pathway analysis indicated the possible function of hsa_circ_0028883 with TB. ROC analysis revealed that hsa_circ_0028883 had potential value for TB diagnosis. Hsa_circ_0028883 is a potentially reliable biomarker to diagnose active TB, but there remains a need to further study the mechanism in TB. |
| 学科主题 | Infectious Diseases |
| WOS关键词 | Tuberculosis ; CircRNA ; CeRNA ; Biomarker |
| 语种 | 英语 |
| WOS记录号 | WOS:000540002900001 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.143/handle/331003/3583]  |
| 专题 | 生化所2019-2020年发文 |
| 作者单位 | 1.Chinese Acad Sci, Univ Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, Shanghai 200031, Peoples R China, 2.Third Peoples Hosp Tibet Autonomous Reg, Dept TB, Lhasa 850000, Peoples R China; 3.Jiangxi Chest Hosp, Dept Crit Care Med, Nanchang 330006, Jiangxi, Peoples R China; 4.East China Univ Sci & Technol, Sch Biotechnol, Shanghai 200237, Peoples R China; 5.Fudan Univ, Shanghai Publ Hlth Clin Ctr, Dept Respirol, Shanghai 201508, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Zhang, Xiaolin,Wu, Qingguo,Tang, Haicheng,et al. Integrated analyses reveal hsa_circ_0028883 as a diagnostic biomarker in active tuberculosis[J]. INFECTION GENETICS AND EVOLUTION,2020,83:104323. |
| APA | Zhang, Xiaolin.,Wu, Qingguo.,Tang, Haicheng.,Zhang, Yunbin.,Li, Feng.,...&,.(2020).Integrated analyses reveal hsa_circ_0028883 as a diagnostic biomarker in active tuberculosis.INFECTION GENETICS AND EVOLUTION,83,104323. |
| MLA | Zhang, Xiaolin,et al."Integrated analyses reveal hsa_circ_0028883 as a diagnostic biomarker in active tuberculosis".INFECTION GENETICS AND EVOLUTION 83(2020):104323. |
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