Membrane rafts-redox signalling pathway contributes to renal fibrosis via modulation of the renal tubular epithelial-mesenchymal transition
文献类型：期刊论文
| 作者 | Han, Wei-Qing1,2,3; Xu, Lian1,2; Tang, Xiao-Feng2,3; Chen, Wen-Dong2,3; Wu, Yong-Jie2,3; Gao, Ping-Jin1,2,3; , |
| 刊名 | JOURNAL OF PHYSIOLOGY-LONDON
 |
| 出版日期 | 2018 |
| 卷号 | 596期号:16页码:3603-3616 |
| 关键词 | membrane rafts epithelial-mesenchymal transition renal fibrosis angiotensin II |
| ISSN号 | 0022-3751 |
| DOI | 10.1113/JP275952 |
| 文献子类 | Article |
| 英文摘要 | The membrane rafts (MRs)-redox pathway is characterized by NADPH oxidase subunit clustering and activation through lysosome fusion, V-type proton ATPase subunit E2 (encoded by the Atp6v1e2 gene) translocation and sphingomyelin phosphodiesterase 1 (SMPD1, encoded by the SMPD1 gene) activation. In the present study, we hypothesized that the MRs-redox-derived reactive oxygen species (ROS) are involved in renal inflammation and fibrosis by promoting renal tubular epithelial-mesenchymal transition (EMT). Results show that transforming growth factor-1 (TGF-1) acutely induced MR formation and ROS production in NRK-52E cells, a rat renal tubular cell line. In addition, transfection of Atp6v1e2 small hairpin RNAs (shRNA) and SMPD1 shRNA attenuated TGF-1-induced changes in EMT markers, including E-cadherin, -smooth muscle actin (-SMA) and fibroblast-specific protein-1 (FSP-1) in NRK-52E cells. Moreover, Erk1/2 activation may be a downstream regulator of the MRs-redox-derived ROS, because both shRNAs significantly inhibited TGF-1-induced Erk1/2 phosphorylation. Further in vivo study shows that the renal tubular the MRs-redox signalling pathway was activated in angiotensin II (AngII)-induced hypertension, as indicated by the increased NADPH oxidase subunit Nox4 fraction in the MR domain, SMPD1 activation and increased ROS content in isolated renal tubular cells. Finally, renal transfection of Atp6v1e2 shRNA and SMPD1 shRNA significantly prevented renal fibrosis and inflammation, as indicated by the decrease of -SMA, fibronectin, collagen I, monocyte chemoattractant protein-1 (MCP-1), intercellular cell adhesion molecule-1 (ICAM-1) and tumour necrosis factor- (TNF-) in kidneys from AngII-infused rats. It was concluded that the the MRs-redox signalling pathway is involved in TGF-1-induced renal tubular EMT and renal inflammation/fibrosis in AngII-induced hypertension. |
| 学科主题 | Neurosciences & Neurology ; Physiology |
| WOS关键词 | ARTERIAL ENDOTHELIAL-CELLS ; PERITONEAL MESOTHELIAL CELLS ; OXIDASE-DEPENDENT FORMATION ; OXYGEN SPECIES CONTRIBUTES ; NADPH OXIDASE ; LYSOSOME FUSION ; ACID SPHINGOMYELINASE ; DIABETIC-NEPHROPATHY ; OXIDATIVE STRESS ; KIDNEY-DISEASE |
| 语种 | 英语 |
| WOS记录号 | WOS:000441707200024 |
| 出版者 | WILEY |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/497]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Hlth Sci, Lab Vasc Biol, Shanghai, Peoples R China; 2.Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, Shanghai Key Lab Hypertens, Shanghai, Peoples R China; 3.Shanghai Res Inst Hypertens, Shanghai, Peoples R China, |
| 推荐引用方式 GB/T 7714 | Han, Wei-Qing,Xu, Lian,Tang, Xiao-Feng,et al. Membrane rafts-redox signalling pathway contributes to renal fibrosis via modulation of the renal tubular epithelial-mesenchymal transition[J]. JOURNAL OF PHYSIOLOGY-LONDON,2018,596(16):3603-3616. |
| APA | Han, Wei-Qing.,Xu, Lian.,Tang, Xiao-Feng.,Chen, Wen-Dong.,Wu, Yong-Jie.,...&,.(2018).Membrane rafts-redox signalling pathway contributes to renal fibrosis via modulation of the renal tubular epithelial-mesenchymal transition.JOURNAL OF PHYSIOLOGY-LONDON,596(16),3603-3616. |
| MLA | Han, Wei-Qing,et al."Membrane rafts-redox signalling pathway contributes to renal fibrosis via modulation of the renal tubular epithelial-mesenchymal transition".JOURNAL OF PHYSIOLOGY-LONDON 596.16(2018):3603-3616. |
入库方式： OAI收割
来源：上海营养与健康研究所
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