Single cell transcriptomic analysis of human mesenchymal stem cells reveals limited heterogeneity
文献类型：期刊论文
| 作者 | Huang, Yin3; Li, Qing3; Hu, Mingyuan3; Wang, Yu3; Du, Liming3; Lin, Liangyu3; Shi, Yufang3; Wang, Ying3; Zhang, Kunshan2; Li, Siguang2 |
| 刊名 | CELL DEATH & DISEASE
 |
| 出版日期 | 2019 |
| 卷号 | 10期号:-页码:UNSP 368 |
| ISSN号 | 2041-4889 |
| 关键词 | Patient-derived xenograft Tumor model Drug response analysis Preclinical drug testing Personalized drug selecting |
| DOI | 10.1038/s41419-019-1583-4 |
| 文献子类 | Article |
| 英文摘要 | Mesenchymal stem cells (MSCs) are a population of multipotent cells with a superior ability to promote tissue repair by regulating regeneration and inflammation. Effective application of MSCs in disease treatment relies on the production of relatively homogeneous cell population. However, the cellular heterogeneity and the differentiation trajectories of in vitro expanded MSCs remain largely unclear. We profiled the transcriptomes of 361 single MSCs derived from two umbilical cords (UC-MSCs). These UC-MSCs were harvested at different passages and stimulated with or without inflammatory cytokines. Weighted gene correlation network analysis revealed that UC-MSCs surprisingly possess only limited heterogeneity, regardless of donors, and passages. We also found that upon pretreatment with inflammatory cytokines (IFN gamma and TNF alpha), a classical strategy that can improve the efficiency of MSC-based therapy, MSCs exhibited uniformed changes in gene expression. Cell cycle-based principal component analysis showed that the limited heterogeneity identified in these UC-MSCs was strongly associated with their entrance into the G2/M phase. This was further proven . by the observation that one featured gene, CD168, was expressed in a cell cycle-dependent manner. When CD168(high) UC-MSCs were sorted and cultured in vitro, they again showed similar CD168 expression patterns. Our results demonstrated that in vitro expanded UC-MSCs are a well-organized population with limited heterogeneity dominated by cell cycle status. Thus, our studies provided information for standardization of MSCs for disease treatment. |
| 学科主题 | Cell Biology |
| WOS关键词 | VERSUS-HOST-DISEASE ; STROMAL CELLS ; MEDIATED IMMUNOSUPPRESSION ; CYCLE ; IDENTIFICATION ; HIERARCHY ; CULTURES |
| 语种 | 英语 |
| 出版者 | NATURE PUBLISHING GROUP |
| WOS记录号 | WOS:000468670100002 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/510]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Univ Munster, Inst Physiol Chem & Pathobiochem, Munster, Germany; 2.Tongji Univ, Tongji Hosp, Translat Stem Cell Res Ctr, Sch Med, Shanghai 200065, Peoples R China; 3.Univ Chinese Acad Sci, Chinese Acad Sci, CAS Key Lab Tissue Microenvironm & Tumor, Shanghai Inst Nutr & Hlth,Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China; 4.Soochow Univ, Inst Translat Med, Suzhou, Peoples R China, 5.Univ Rome, Dept Expt Med & Surg, Biochem Lab IDI IRCC, I-00133 Rome, Italy; |
| 推荐引用方式 GB/T 7714 | Huang, Yin,Li, Qing,Hu, Mingyuan,et al. Single cell transcriptomic analysis of human mesenchymal stem cells reveals limited heterogeneity[J]. CELL DEATH & DISEASE,2019,10(-):UNSP 368. |
| APA | Huang, Yin.,Li, Qing.,Hu, Mingyuan.,Wang, Yu.,Du, Liming.,...&,.(2019).Single cell transcriptomic analysis of human mesenchymal stem cells reveals limited heterogeneity.CELL DEATH & DISEASE,10(-),UNSP 368. |
| MLA | Huang, Yin,et al."Single cell transcriptomic analysis of human mesenchymal stem cells reveals limited heterogeneity".CELL DEATH & DISEASE 10.-(2019):UNSP 368. |
入库方式： OAI收割
来源：上海营养与健康研究所
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