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A dual genetic tracing system identifies diverse and dynamic origins of cardiac valve mesenchyme

文献类型：期刊论文

作者Liu, Kuo4,5; Yu, Wei4,5; Tang, Juan4,5; Liu, Xiuxiu5; Liu, Qiaozhen4,5; Li, Yan4,5; He, Lingjuan4,5; Zhang, Libo4,5; Zhou, Bin2,4,5; Tang, Muxue3
刊名DEVELOPMENT
出版日期2018
卷号145期号:18页码:UNSP dev167775
关键词Genetic engineering Site-specific recombinase (SSR) Nigri-nox Fate mapping Cardiac valve development Genetic engineering Site-specific recombinase (SSR) Nigri-nox Fate mapping Cardiac valve development
ISSN号0950-1991
DOI10.1242/dev.167775
文献子类Article
英文摘要In vivo genomic engineering is instrumental for studying developmental biology and regenerative medicine. Development of novel systems with more site-specific recombinases (SSRs) that complement with the commonly used Cre-loxP would be valuable for more precise lineage tracing and genome editing. Here, we introduce a new SSR system via Nigri-nox. By generating tissue-specific Nigri knock-in and its responding nox reporter mice, we show that the Nigri-nox system works efficiently in vivo by targeting specific tissues. As a new orthogonal system to Cre-loxP, Nigri-nox provides an additional control of genetic manipulation. We also demonstrate how the two orthogonal systems Nigri-nox and Cre-loxP could be used simultaneously to map the cell fate of two distinct developmental origins of cardiac valve mesenchyme in the mouse heart, providing dynamics of cellular contribution from different origins for cardiac valve mesenchyme during development. This work provides a proofof-principle application of the Nigri-nox system for in vivo mouse genomic engineering. Coupled with other SSR systems, Nigri-nox would be valuable for more precise delineation of origins and cell fates during development, diseases and regeneration.
学科主题Developmental Biology
WOS关键词SITE-SPECIFIC RECOMBINASE ; NEURAL CREST ; EPICARDIAL PROGENITORS ; MAMMALIAN-CELLS ; STEM-CELLS ; LINEAGE ; HEART ; MICE ; EXPRESSION ; FATE
语种英语
WOS记录号WOS:000447270800014
出版者COMPANY BIOLOGISTS LTD
版本出版稿
源URL[http://202.127.25.144/handle/331004/513]  
专题中国科学院上海生命科学研究院营养科学研究所
作者单位1.Chinese Univ Hong Kong, Prince Wales Hosp, Dept Chem Pathol, Li Ka Shing Inst Hlth Sci, Shatin, Hong Kong, Peoples R China;
2.Jinan Univ, Key Lab Regenerat Med, Minist Educ, Guangzhou 510632, Guangdong, Peoples R China;
3.East China Normal Univ, Sch Life Sci, Shanghai 200241, Peoples R China;
4.Univ Chinese Acad Sci, Chinese Acad Sci, Inst Nutr Sci, Key Lab Nutr & Metab,Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China;
5.Univ Chinese Acad Sci, Chinese Acad Sci, CAS Ctr Excellence Mol Cell Sci, State Key Lab Cell Biol,Shanghai Inst Biochem & C, Shanghai 200031, Peoples R China;
6.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai 201210, Peoples R China,
7.Univ Calif San Diego, Skaggs Sch Pharm & Pharmaceut Sci, La Jolla, CA 92093 USA;
推荐引用方式
GB/T 7714		 Liu, Kuo,Yu, Wei,Tang, Juan,et al. A dual genetic tracing system identifies diverse and dynamic origins of cardiac valve mesenchyme[J]. DEVELOPMENT,2018,145(18):UNSP dev167775.
APA Liu, Kuo.,Yu, Wei.,Tang, Juan.,Liu, Xiuxiu.,Liu, Qiaozhen.,...&,.(2018).A dual genetic tracing system identifies diverse and dynamic origins of cardiac valve mesenchyme.DEVELOPMENT,145(18),UNSP dev167775.
MLA Liu, Kuo,et al."A dual genetic tracing system identifies diverse and dynamic origins of cardiac valve mesenchyme".DEVELOPMENT 145.18(2018):UNSP dev167775.

入库方式： OAI收割

来源：上海营养与健康研究所

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