A vitamin-C-derived DNA modification catalysed by an algal TET homologue
文献类型：期刊论文
| 作者 | Xue, Jian-Huang17; Chen, Guo-Dong17; Chen, Hui17; Fang, Zhaoyuan17; Yang, Qing-Lin17; Fan, Qiang-Qiang17; Wang, Bang-An17; Zhang, Xiao-Jie17; Tang, Hui17; Chen, Luonan11,17 |
| 刊名 | NATURE
 |
| 出版日期 | 2019 |
| 卷号 | 569期号:7757页码:581-+ |
| ISSN号 | 0028-0836 |
| 关键词 | Asthma group 2 innate lymphoid cells IL-17 |
| DOI | 10.1038/s41586-019-1160-0 |
| 文献子类 | Article |
| 英文摘要 | Methylation of cytosine to 5-methylcytosine (5mC) is a prevalent DNA modification found in many organisms. Sequential oxidation of 5mC by ten-eleven translocation (TET) dioxygenases results in a cascade of additional epigenetic marks and promotes demethylation of DNA in mammals(1,2). However, the enzymatic activity and function of TET homologues in other eukaryotes remains largely unexplored. Here we show that the green alga Chlamydomonas reinhardtii contains a 5mC-modifying enzyme (CMD1) that is a TET homologue and catalyses the conjugation of a glyceryl moiety to the methyl group of 5mC through a carbon-carbon bond, resulting in two stereoisomeric nucleobase products. The catalytic activity of CMD1 requires Fe(ii) and the integrity of its binding motif His-XAsp, which is conserved in Fe-dependent dioxygenases(3). However, unlike previously described TET enzymes, which use 2-oxoglutarate as a co-substrate(4), CMD1 uses l-ascorbic acid (vitamin C) as an essential co-substrate. Vitamin C donates the glyceryl moiety to 5mC with concurrent formation of glyoxylic acid and CO2. The vitamin-C-derived DNA modification is present in the genome of wild-type C. reinhardtii but at a substantially lower level in a CMD1 mutant strain. The fitness of CMD1 mutant cells during exposure to high light levels is reduced. LHCSR3, a gene that is critical for the protection of C. reinhardtii from photo-oxidative damage under high light conditions, is hypermethylated and downregulated in CMD1 mutant cells compared to wild-type cells, causing a reduced capacity for photoprotective non-photochemical quenching. Our study thus identifies a eukaryotic DNA base modification that is catalysed by a divergent TET homologue and unexpectedly derived from vitamin C, and describes its role as a potential epigenetic mark that may counteract DNA methylation in the regulation of photosynthesis. |
| 学科主题 | Science & Technology - Other Topics |
| WOS关键词 | CHLAMYDOMONAS-REINHARDTII ; ASCORBATE BIOSYNTHESIS ; EXPRESSION ANALYSIS ; 5-METHYLCYTOSINE ; GENE ; 5-HYDROXYMETHYLCYTOSINE ; DEMETHYLATION ; 5-FORMYLCYTOSINE ; MECHANISMS ; PROTEINS |
| 语种 | 英语 |
| 出版者 | NATURE PUBLISHING GROUP |
| WOS记录号 | WOS:000468844100056 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/518]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Fudan Univ, State Key Lab Genet Engn, Zhongshan Hosp, Shanghai, Peoples R China; 2.Univ Penn, Dept Biochem & Biophys, Perelman Sch Med, Philadelphia, PA 19104 USA; 3.Fudan Univ, Key Lab Med Epigenet & Metab, Inst Biomed Sci, Shanghai Med Coll, Shanghai, Peoples R China, 4.Univ Penn, Dept Med, Perelman Sch Med, Philadelphia, PA 19104 USA; 5.Rhein Westfal TH Aachen, Inst Organ Chem, Aachen, Germany; 6.Chinese Acad Sci, Natl Ctr Prot Sci Shanghai, Inst Biochem & Cell Biol, Shanghai, Peoples R China; 7.Chinese Acad Sci, Ctr Excellence Anim Evolut & Genet, Kunming, Yunnan, Peoples R China; 8.Chinese Acad Sci, Key Lab Algal Biol, Inst Hydrobiol, Wuhan, Hubei, Peoples R China; 9.Shanghai Normal Univ, Coll Life Sci, Shanghai, Peoples R China; 10.Chinese Acad Sci, Key Lab Computat Biol, Chinese Acad Sci German Max Planck Soc Partner In, Shanghai Inst Biol Sci, Shanghai, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Xue, Jian-Huang,Chen, Guo-Dong,Chen, Hui,et al. A vitamin-C-derived DNA modification catalysed by an algal TET homologue[J]. NATURE,2019,569(7757):581-+. |
| APA | Xue, Jian-Huang.,Chen, Guo-Dong.,Chen, Hui.,Fang, Zhaoyuan.,Yang, Qing-Lin.,...&,.(2019).A vitamin-C-derived DNA modification catalysed by an algal TET homologue.NATURE,569(7757),581-+. |
| MLA | Xue, Jian-Huang,et al."A vitamin-C-derived DNA modification catalysed by an algal TET homologue".NATURE 569.7757(2019):581-+. |
入库方式： OAI收割
来源：上海营养与健康研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。