Activin/Smad2-induced Histone H3 Lys-27 Trimethylation (H3K27me3) Reduction Is Crucial to Initiate Mesendoderm Differentiation of Human Embryonic Stem Cells
文献类型：期刊论文
| 作者 | Wang, Lu3; Xu, Xuanhao3; Li, Zhongwei3; Zhu, Gaoyang3; Chen, Ye-Guang3; Cao, Yaqiang4; Cheng, Hao4; Han, Jing-Dong J.4; Duan, Fuyu1; Na, Jie1 |
| 刊名 | JOURNAL OF BIOLOGICAL CHEMISTRY
 |
| 出版日期 | 2017 |
| 卷号 | 292期号:4页码:1339-+ |
| 关键词 | calcium silicate chitosan cardiac patch bioactive ions aligned nanostructure |
| ISSN号 | 0021-9258 |
| DOI | 10.1074/jbc.M116.766949 |
| 文献子类 | Article |
| 英文摘要 | Differentiation of human embryonic stem cells into mesendoderm (ME) is directed by extrinsic signals and intrinsic epigenetic modifications. However, the dynamics of these epigenetic modifications and the mechanisms by which extrinsic signals regulate the epigenetic modifications during the initiation of ME differentiation remain elusive. In this study, we report that levels of histone H3 Lys-27 trimethylation (H3K27me3) decrease during ME initiation, which is essential for subsequent differentiation induced by the combined effects of activin and Wnt signaling. Furthermore, we demonstrate that activin mediates the H3K27me3 decrease via the Smad2-mediated reduction of EZH2 protein level. Our results suggest a two-step process of ME initiation: first, epigenetic priming via removal of H3K27me3 marks and, second, transcription activation. Our findings demonstrate a critical role of H3K27me3 priming and a direct interaction between extrinsic signals and epigenetic modifications during ME initiation. |
| 学科主题 | Biochemistry & Molecular Biology |
| WOS关键词 | TGF-BETA ; ENDODERM DIFFERENTIATION ; DEMETHYLASE JMJD3 ; MOUSE DEVELOPMENT ; DNA METHYLATION ; SELF-RENEWAL ; TARGET GENES ; IN-VITRO ; HUMAN ES ; TRANSCRIPTION |
| 语种 | 英语 |
| WOS记录号 | WOS:000393339800016 |
| 出版者 | AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/519]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Tsinghua Univ, Sch Med, Beijing 100084, Peoples R China; 2.Salk Inst Biol Studies, Gene Express Lab, La Jolla, CA 92037 USA, 3.Tsinghua Univ, Sch Life Sci, Tsinghua Peking Ctr Life Sci, State Key Lab Membrane Biol, Beijing 100084, Peoples R China; 4.Chinese Acad Sci, Shanghai Inst Biol Sci, Chinese Acad Sci Max Planck Partner Inst Computat, Key Lab Computat Biol, 320 Yue Yang Rd, Shanghai 200031, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Wang, Lu,Xu, Xuanhao,Li, Zhongwei,et al. Activin/Smad2-induced Histone H3 Lys-27 Trimethylation (H3K27me3) Reduction Is Crucial to Initiate Mesendoderm Differentiation of Human Embryonic Stem Cells[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2017,292(4):1339-+. |
| APA | Wang, Lu.,Xu, Xuanhao.,Li, Zhongwei.,Zhu, Gaoyang.,Chen, Ye-Guang.,...&,.(2017).Activin/Smad2-induced Histone H3 Lys-27 Trimethylation (H3K27me3) Reduction Is Crucial to Initiate Mesendoderm Differentiation of Human Embryonic Stem Cells.JOURNAL OF BIOLOGICAL CHEMISTRY,292(4),1339-+. |
| MLA | Wang, Lu,et al."Activin/Smad2-induced Histone H3 Lys-27 Trimethylation (H3K27me3) Reduction Is Crucial to Initiate Mesendoderm Differentiation of Human Embryonic Stem Cells".JOURNAL OF BIOLOGICAL CHEMISTRY 292.4(2017):1339-+. |
入库方式： OAI收割
来源：上海营养与健康研究所
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