Biodegradation of mycotoxin fumonisin B1 by a novel bacterial consortium SAAS79
文献类型：期刊论文
| 作者 | Zhao, Zhiyong1; Zhang, Yanmei1; Chen, Shanshan1; Zhao, Xiaoyan1; Li, Xiaobei1; Chen, Lei1; Zhou, Changyan1; Wang, Jianhua1; Gong, Andong2; Liu, Na3 |
| 刊名 | APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
 |
| 出版日期 | 2019 |
| 卷号 | 103期号:17页码:7129-7140 |
| 关键词 | Biodegradation Fumonisin B1 Bacterial consortium Enzymatic detoxification Metabolites |
| ISSN号 | 0175-7598 |
| DOI | 10.1007/s00253-019-09979-6 |
| 文献子类 | Article |
| 英文摘要 | Fumonisin B1 (FB1) contamination in cereals and cereal products remains an important aspect of food safety because of its wide distribution and the potential health hazard. However, only a few microorganisms have been reported to effectively degrade FB1. In this present study, a bacterial consortium SAAS79 with highly FB1-degrading activity was isolated from the spent mushroom compost. The combination of antibiotic-driven selection and 16S rDNA sequencing identified the Pseudomonas genus as the key FB1-degrading member. The microbial consortium could degrade more than 90% of 10 mu g/mL FB1 after incubation for 24 h at pH of 5-7 and temperature of 28-35 degrees C. The enzymes from the intracellular space were proved to be responsible for FB1 degradation, which eliminated about 90% of 10 mu g/mL FB1 in 3 h. Besides, liquid chromatography time-of-flight mass spectrometry (LC-TOF/MS) analysis identified two degradation products of FB1, and their toxicity on the monkey kidney cells (MARC-145) was significantly lower (p < 0.05) compared with the parent FB1. Overall, the consortium SAAS79 and its crude enzymes may be a potential choice for the decontamination of FB1 in the feed and food industry. Also, the bacterial consortium provides a new source of genes for the development of enzymatic detoxification agent. |
| 学科主题 | Biotechnology & Applied Microbiology |
| WOS关键词 | AFLATOXIN B-1 DEGRADATION ; DETOXIFICATION ; METABOLISM ; MECHANISM ; CULTURE ; FEED ; FOOD ; FB1 |
| 语种 | 英语 |
| WOS记录号 | WOS:000480552400019 |
| 出版者 | SPRINGER |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/520]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Shanghai Acad Agr Sci, Minist Agr, Lab Qual & Safety Risk Assessment Agroprod Shangh, Inst Agrofood Stand & Testing Technol, 1000 Jinqi Rd, Shanghai 201403, Peoples R China; 2.Xinyang Normal Univ, Coll Life & Sci, Xinyang 464000, Peoples R China; 3.Univ Chinese Acad Sci, Chinese Acad Sci, CAS Key Lab Nutr Metab & Food Safety, Shanghai Inst Nutr & Hlth,Shanghai Inst Biol Sci, Huairou, Peoples R China, |
| 推荐引用方式 GB/T 7714 | Zhao, Zhiyong,Zhang, Yanmei,Chen, Shanshan,et al. Biodegradation of mycotoxin fumonisin B1 by a novel bacterial consortium SAAS79[J]. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,2019,103(17):7129-7140. |
| APA | Zhao, Zhiyong.,Zhang, Yanmei.,Chen, Shanshan.,Zhao, Xiaoyan.,Li, Xiaobei.,...&,.(2019).Biodegradation of mycotoxin fumonisin B1 by a novel bacterial consortium SAAS79.APPLIED MICROBIOLOGY AND BIOTECHNOLOGY,103(17),7129-7140. |
| MLA | Zhao, Zhiyong,et al."Biodegradation of mycotoxin fumonisin B1 by a novel bacterial consortium SAAS79".APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 103.17(2019):7129-7140. |
入库方式： OAI收割
来源：上海营养与健康研究所
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