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Age at menarche and age at natural menopause in East Asian women: a genome-wide association study (vol 38, pg 513, 2016)

文献类型：期刊论文

作者Shi, Jiajun3,4; Zhang, Ben3,4; Long, Jirong3,4; Wen, Wanqing3,4; Cai, Qiuyin3,4; Shu, Xiao-Ou3,4; Choi, Ji-Yeob1,9,10; Kang, Daehee1,9,10; Park, Sue K.1,9,10; Chung, Seokang10
刊名GEROSCIENCE
出版日期2018
卷号40期号:1页码:71-72
关键词Berberine Nonalcoholic fatty liver disease NAFLD Stearyl-coenzyme A desaturase 1 SCD1 Sterol regulatory element-binding protein-1c SREBP-1c AMP-activated protein kinase AMPK
ISSN号2509-2715
DOI10.1007/s11357-018-0007-1
文献子类Correction
英文摘要Background: Berberine (BBR), a natural compound extracted from Chinese herb, has been shown to effectively attenuate nonalcoholic fatty liver disease (NAFLD) in clinic. However, the mechanism underlying the effect of BBR is not fully understood. Stearyl-coenzyme A desaturase 1 (SCD1) mediates lipid metabolism in liver. Therefore, we hypothesized that SCD1 mediated the beneficial effect of BBR on NAFLD. Methods: The expression of SCD1 was measured in the liver of NAFLD patients and ob/ob mice. The effect of BBR on NAFLD was evaluated in C57BL/6 J mice on high fat diet (HFD). The effect of BBR was also investigated in HepG2 and AML12 cells exposed to high glucose and palmitic acid. Oil red O staining was performed to detect triglyceride (TG) level. Quantitative real-time polymerase chain reaction and Western blot were used to detect the messenger ribonucleic acid (mRNA) and protein expression of target genes. The activity of SCD1 promoter was measured by dual-luciferase reporter assay. Results: The expression of SCD1 was increased in the liver of NAFLD patients and ob/ob mice. BBR reduced hepatic TG accumulation and decreased the expressions of hepatic SCD1 and other TG synthesis related genes both in vivo and in vitro. Knockdown of SCD1 expression mimicked the effect of BBR decreasing TG level in steatotic hepatocytes, whereas overexpression of SCD1 attenuated the effect of BBR. Mechanistically, BBR promoted the phosphorylation of AMP-activated protein kinase (AMPK) and sterol regulatory element-binding protein-1c (SREBP-1c) in HepG2 cells and the liver of HFD-fed mice. Activation of the AMPK-SREBP-lc pathway and sterol regulatory element (SRE) motif in SCD1 promoter (-920/-550) was responsible for the BBR-induced suppression of SCD1. Conclusion: BBR reduces liver TG synthesis and attenuates hepatic steatosis through the activation of AMPK-SREBP-1c-SCD1 pathway.
学科主题Geriatrics & Gerontology
语种英语
WOS记录号WOS:000426572000006
出版者SPRINGER
版本出版稿
源URL[http://202.127.25.144/handle/331004/530]  
专题中国科学院上海生命科学研究院营养科学研究所
作者单位1.Seoul Natl Univ, Canc Res Inst, Seoul, South Korea;
2.Shanghai Jiao Tong Univ, Sch Med, Dept Epidemiol, Shanghai Canc Inst,Renji Hosp, Shanghai, Peoples R China;
3.Vanderbilt Univ, Sch Med, Dept Med, IMPH,Vanderbilt Epidemiol Ctr, 2525 West End Ave,Suite 600, Nashville, TN 37203 USA;
4.Vanderbilt Univ, Sch Med, Div Epidemiol, IMPH, 2525 West End Ave,Suite 600, Nashville, TN 37203 USA;
5.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Nutr Sci, Key Lab Nutr & Metab, Shanghai, Peoples R China;
6.Chinese Acad Sci, Grad Sch, Shanghai, Peoples R China;
7.Seoul Natl Univ, Coll Med, Dept Surg, Seoul, South Korea,
8.Shanghai Municipal Ctr Dis Control & Prevent, Shanghai, Peoples R China;
9.Seoul Natl Univ, Coll Med, Dept Prevent Med, Seoul, South Korea;
10.Seoul Natl Univ, Dept Biomed Sci, Coll Med, Seoul, South Korea;
推荐引用方式
GB/T 7714		 Shi, Jiajun,Zhang, Ben,Long, Jirong,et al. Age at menarche and age at natural menopause in East Asian women: a genome-wide association study (vol 38, pg 513, 2016)[J]. GEROSCIENCE,2018,40(1):71-72.
APA Shi, Jiajun.,Zhang, Ben.,Long, Jirong.,Wen, Wanqing.,Cai, Qiuyin.,...&,.(2018).Age at menarche and age at natural menopause in East Asian women: a genome-wide association study (vol 38, pg 513, 2016).GEROSCIENCE,40(1),71-72.
MLA Shi, Jiajun,et al."Age at menarche and age at natural menopause in East Asian women: a genome-wide association study (vol 38, pg 513, 2016)".GEROSCIENCE 40.1(2018):71-72.

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来源：上海营养与健康研究所

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