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Heat shock protein DNAJA1 stabilizes PIWI proteins to support regeneration and homeostasis of planarian Schmidtea mediterranea

文献类型：期刊论文

作者Wang, Chen2,3; Yang, Zhen-Zhen3; Shi, Shuo3; Lin, Haifan3; Guo, Fang-Hao2; Han, Xiao-Shuai2; Zeng, An2; Jing, Qing2; Lin, Haifan1; ,
刊名JOURNAL OF BIOLOGICAL CHEMISTRY
出版日期2019
卷号294期号:25页码:9873-9887
关键词regeneration heat shock protein (HSP) stem cells chaperone Argonaute DNAJA1 heat shock protein 40 (Hsp40) neoblast PIWI proteins planarian PIWI-interacting RNA (piRNA)
ISSN号1671-4083
DOI10.1074/jbc.RA118.004445
文献子类Article
英文摘要PIWI proteins are key regulators of germline and somatic stem cells throughout different evolutionary lineages. However, how PIWI proteins themselves are regulated remains largely unknown. To identify candidate proteins that interact with PIWI proteins and regulate their stability, here we established a yeast two-hybrid (Y2H) assay in the planarian species Schmidtea mediterranea. We show that DNAJA1, a heat shock protein 40 family member, interacts with the PIWI protein SMEDWI-2, as validated by the Y2H screen and co-immunoprecipitation assays. We found that DNAJA1 is enriched in planarian adult stem cells, the nervous system, and intestinal tissues. DNAJA1-knockdown abolished planarian regeneration and homeostasis, compromised stem cell maintenance and PIWI-interacting RNA (piRNA) biogenesis, and deregulated SMEDWI-1/2 target genes. Mechanistically, we observed that DNAJA1 is required for the stability of SMEDWI-1 and SMEDWI-2 proteins. Furthermore, we noted that human DNAJA1 binds to Piwi-like RNA-mediated gene silencing 1 (PIWIL1) and is required for PIWIL1 stability in human gastric cancer cells. In summary, our results reveal not only an evolutionarily conserved functional link between PIWI and DNAJA1 that is essential for PIWI protein stability and piRNA biogenesis, but also an important role of DNAJA1 in the control of proteins involved in stem cell regulation.
学科主题Biochemistry & Molecular Biology
WOS关键词PLURIPOTENT STEM-CELLS ; PIRNA BIOGENESIS ; CHAPERONE MACHINERY ; SELF-RENEWAL ; DROSOPHILA ; EXPRESSION ; GERMLINE ; FAMILY ; GENES ; TRANSPOSONS
语种英语
WOS记录号WOS:000473277900019
出版者AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
版本出版稿
源URL[http://202.127.25.144/handle/331004/558]  
专题中国科学院上海生命科学研究院营养科学研究所
作者单位1.Yale Sch Med, Yale Stem Cell Ctr, New Haven, CT 06511 USA,
2.Univ Chinese Acad Sci, Chinese Acad Sci, CAS Key Lab Tissue Microenvironm & Tumor, Shanghai Inst Nutr & Hlth,Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China;
3.ShanghaiTech Univ, Shanghai Inst Adv Immunochem Studies, Shanghai 201210, Peoples R China;
推荐引用方式
GB/T 7714		 Wang, Chen,Yang, Zhen-Zhen,Shi, Shuo,et al. Heat shock protein DNAJA1 stabilizes PIWI proteins to support regeneration and homeostasis of planarian Schmidtea mediterranea[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2019,294(25):9873-9887.
APA Wang, Chen.,Yang, Zhen-Zhen.,Shi, Shuo.,Lin, Haifan.,Guo, Fang-Hao.,...&,.(2019).Heat shock protein DNAJA1 stabilizes PIWI proteins to support regeneration and homeostasis of planarian Schmidtea mediterranea.JOURNAL OF BIOLOGICAL CHEMISTRY,294(25),9873-9887.
MLA Wang, Chen,et al."Heat shock protein DNAJA1 stabilizes PIWI proteins to support regeneration and homeostasis of planarian Schmidtea mediterranea".JOURNAL OF BIOLOGICAL CHEMISTRY 294.25(2019):9873-9887.

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来源：上海营养与健康研究所

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