Human cleaving embryos enable robust homozygotic nucleotide substitutions by base editors
文献类型：期刊论文
| 作者 | Zhang, Meiling1,7; Sun, Yun1,7; Yao, Ning1,7; Zhong, Wanxia1,7; Li, Yun1,7; Chen, Zi-jiang1,2,3,4,7; Zhou, Changyang5,9; Wei, Yu9; Xu, Chunlong9; Pan, Hong9 |
| 刊名 | GENOME BIOLOGY
 |
| 出版日期 | 2019 |
| 卷号 | 20期号:-页码:101 |
| 关键词 | Base editing Human cleaving embryos Homozygotic nucleotide substitution |
| ISSN号 | 1474-760X |
| DOI | 10.1186/s13059-019-1703-6 |
| 文献子类 | Article |
| 英文摘要 | Base editing installs a precise nucleotide change in specific gene loci without causing a double-strand break. Its efficiency in human embryos is generally low, limiting its utility in functional genetic studies. Here, we report that injecting base editors into human cleaving two-cell and four-cell embryos results in much higher (up to 13-fold) homozygotic nucleotide substitution efficiency as opposed to MII oocytes or zygotes. Furthermore, as a proof-of-principle study, a point mutation can be efficiently corrected by our method. Our study indicates that human cleaving embryos provide an efficient base editing window for robust gene disruption and correction. |
| 学科主题 | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:000468850400002 |
| 出版者 | BMC |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/565]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Shanghai Jiao Tong Univ, Ren Ji Hosp, Ctr Reprod Med, Sch Med, Shanghai, Peoples R China; 2.Shandong Univ, Ctr Reprod Med, Shandong Prov Hosp, Jinan, Shandong, Peoples R China; 3.Natl Res Ctr Assisted Reprod Technol & Reprod Gen, Jinan, Shandong, Peoples R China; 4.Minist Educ, Key Lab Reprod Endocrinol, Jinan 250021, Shandong, Peoples R China; 5.Univ Chinese Acad Sci, Coll Life Sci, Beijing 100049, Peoples R China; 6.Chinese Acad Sci, CAS MPG Partner Inst Computat Biol, Shanghai Inst Biol Sci, Key Lab Computat Biol, Shanghai 200031, Peoples R China, 7.Shanghai Key Lab Assisted Reprod & Reprod Genet, Shanghai 200127, Peoples R China; 8.Oregon Hlth & Sci Univ, Ctr Embryon Cell & Gene Therapy, 3303 Southwest,Bond Ave, Portland, OR 97239 USA; 9.Chinese Acad Sci, Shanghai Inst Biol Sci, CAS Ctr Excellence Brain Sci & Intelligence Techn, Inst Neurosci,State Key Lab Neurosci,Key Lab Prim, Shanghai 200031, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Zhang, Meiling,Sun, Yun,Yao, Ning,et al. Human cleaving embryos enable robust homozygotic nucleotide substitutions by base editors[J]. GENOME BIOLOGY,2019,20(-):101. |
| APA | Zhang, Meiling.,Sun, Yun.,Yao, Ning.,Zhong, Wanxia.,Li, Yun.,...&,.(2019).Human cleaving embryos enable robust homozygotic nucleotide substitutions by base editors.GENOME BIOLOGY,20(-),101. |
| MLA | Zhang, Meiling,et al."Human cleaving embryos enable robust homozygotic nucleotide substitutions by base editors".GENOME BIOLOGY 20.-(2019):101. |
入库方式： OAI收割
来源：上海营养与健康研究所
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