Schistosoma japonicum extracellular vesicle miRNA cargo regulates host macrophage functions facilitating parasitism
文献类型：期刊论文
| 作者 | Liu, Juntao3; Zhu, Lihui3; Chen, Yongjun3; Cheng, Guofeng3; Wang, Jianbin1,2; Davis, Richard E.1,2; Qiu, Lin4; , |
| 刊名 | PLOS PATHOGENS
 |
| 出版日期 | 2019 |
| 卷号 | 15期号:6页码:e1007817 |
| 关键词 | Hepatocyte-like cells Human pluripotent stem cells Hepatic differentiation Biomedical application |
| ISSN号 | 1553-7366 |
| DOI | 10.1371/journal.ppat.1007817 |
| 文献子类 | Article |
| 英文摘要 | Schistosome infection persists for decades. Parasites are in close contact with host peripheral blood immune cells, yet little is known about the regulatory interactions between parasites and these immune cells. Here, we report that extracellular vesicles (EVs) released from Schistosoma japonicum are taken up primarily by macrophages and other host peripheral blood immune cells and their miRNA cargo transferred into recipient cells. Uptake of S. japonicum EV miR-125b and bantam miRNAs into host cells increased macrophage proliferation and TNF-alpha production by regulating the corresponding targets including Pros1, Fam212b, and Clmp. Mice infected with S. japonicum exhibit an increased population of monocytes and elevated levels of TNF-alpha. Reduction of host monocytes and TNF-alpha level in S. japonicum infected mice led to a significant reduction in worm and egg burden and pathology. Overall, we demonstrate that S. japonicum EV miRNAs can regulate host macrophages illustrating parasite modulation of the host immune response to facilitate parasite survival. Our findings provide valuable insights into the schistosome-host interaction which may help to develop novel intervention strategies against schistosomiasis. Author summary Schistosomes that cause schistosomiasis infection persist for decades despite a host immune response. Therefore, elucidating the mechanism of schistosome survival will not only contribute to the understanding of host-parasite interaction but also lead to the development of novel strategies against schistosomiasis. Extracellular vesicles (EVs) and their miRNA cargo have been shown to be mediators of intercellular communication involved in the regulation of many biological processes. Here, we demonstrated that EVs released from Schistosoma japonicum (SjEVs) are taken up primarily by macrophages and other host peripheral blood immune cells and their miRNA cargo transferred into recipient cells. Uptake of S. japonicum EV miR-125b and bantam miRNAs into host cells increased macrophage proliferation and TNF-alpha production that contributes to parasite survival. Our findings reveal key roles of SjEV miRNAs for facilitating parasitism in schistosomes. |
| 学科主题 | Microbiology ; Parasitology ; Virology |
| WOS关键词 | NECROSIS-FACTOR-ALPHA ; TGF-BETA ; TNF-ALPHA ; MICE LACKING ; MANSONI ; EXPRESSION ; COMMUNICATION ; APOPTOSIS ; REVEALS ; VACCINE |
| 语种 | 英语 |
| WOS记录号 | WOS:000479154700024 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/567]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Univ Colorado, Sch Med, RNA Biosci Initiat, Dept Biochem, Aurora, CO USA; 2.Univ Colorado, Sch Med, RNA Biosci Initiat, Dept Mol Genet, Aurora, CO USA; 3.Chinese Acad Agr Sci, Shanghai Vet Res Inst, Key Lab Anim Parasitol, Minist Agr, Shanghai, Peoples R China; 4.Chinese Acad Sci, Shanghai Inst Nutr & Hlth, Shanghai, Peoples R China, |
| 推荐引用方式 GB/T 7714 | Liu, Juntao,Zhu, Lihui,Chen, Yongjun,et al. Schistosoma japonicum extracellular vesicle miRNA cargo regulates host macrophage functions facilitating parasitism[J]. PLOS PATHOGENS,2019,15(6):e1007817. |
| APA | Liu, Juntao.,Zhu, Lihui.,Chen, Yongjun.,Cheng, Guofeng.,Wang, Jianbin.,...&,.(2019).Schistosoma japonicum extracellular vesicle miRNA cargo regulates host macrophage functions facilitating parasitism.PLOS PATHOGENS,15(6),e1007817. |
| MLA | Liu, Juntao,et al."Schistosoma japonicum extracellular vesicle miRNA cargo regulates host macrophage functions facilitating parasitism".PLOS PATHOGENS 15.6(2019):e1007817. |
入库方式： OAI收割
来源：上海营养与健康研究所
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