Degrading Ochratoxin A and Zearalenone Mycotoxins Using a Multifunctional Recombinant Enzyme
文献类型：期刊论文
| 作者 | Azam, Md Shofiul; Yu, Dianzhen; Liu, Na; Wu, Aibo; , |
| 刊名 | TOXINS
 |
| 出版日期 | 2019 |
| 卷号 | 11期号:5页码:301 |
| 关键词 | fusion enzyme ochratoxin A carboxypeptidase zearalenone ZHD101 HK293 cell LX2 cell |
| ISSN号 | 2072-6651 |
| DOI | 10.3390/toxins11050301 |
| 文献子类 | Article |
| 英文摘要 | Zearalenone (ZEA) is an estrogenic and ochratoxin A (OTA) is a hepatotoxic Fusarium mycotoxin commonly seen in cereals and fruits products. No previous investigation has studied on a single platform for the multi degradation mycotoxin. The current study aimed to investigate the bifunctional activity of a novel fusion recombinant. We have generated a recombinant fusion enzyme (ZHDCP) by combining two single genes named zearalenone hydrolase (ZHD) and carboxypeptidase (CP) in frame deletion by crossover polymerase chain reaction (PCR). We identified enzymatic properties and cell cytotoxicity assay of ZHDCP enzyme. Our findings have demonstrated that ZEA was completely degraded to the non-toxic product in 2 h by ZHDCP enzyme at an optimum pH of 7 and a temperature of 35 degrees C. For the first time, it was found out that ZEA 60% was degraded by CP degrades in 48 h. Fusion ZHDCP and CP enzyme were able to degrade 100% OTA in 30 min at pH 7 and temperature 30 degrees C. ZEA- and OTA-induced cell death and increased cell apoptosis rate and regulated mRNA expression of Sirt1, Bax, Bcl2, Caspase3, TNF, and IL6 genes. Our novel findings demonstrated that the fusion enzyme ZHDCP possess bifunctional activity (degrade OTA and ZEA), and it could be used to degrade more mycotoxins. |
| 学科主题 | Food Science & Technology ; Toxicology |
| WOS关键词 | DEGRADATION ; DETOXIFICATION ; BIODEGRADATION ; CYTOTOXICITY ; METABOLISM ; TOXICITY ; DESIGN ; CELLS ; FEED ; FOOD |
| 语种 | 英语 |
| WOS记录号 | WOS:000472672300064 |
| 出版者 | MDPI |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/571]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | Univ Chinese Acad Sci, SIBS UGENT SJTU Joint Lab Mycotoxin Res, CAS Key Lab Nutr Metab & Food Safety,Chinese Acad, Shanghai Inst Nutr & Hlth,Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China, |
| 推荐引用方式 GB/T 7714 | Azam, Md Shofiul,Yu, Dianzhen,Liu, Na,et al. Degrading Ochratoxin A and Zearalenone Mycotoxins Using a Multifunctional Recombinant Enzyme[J]. TOXINS,2019,11(5):301. |
| APA | Azam, Md Shofiul,Yu, Dianzhen,Liu, Na,Wu, Aibo,&,.(2019).Degrading Ochratoxin A and Zearalenone Mycotoxins Using a Multifunctional Recombinant Enzyme.TOXINS,11(5),301. |
| MLA | Azam, Md Shofiul,et al."Degrading Ochratoxin A and Zearalenone Mycotoxins Using a Multifunctional Recombinant Enzyme".TOXINS 11.5(2019):301. |
入库方式： OAI收割
来源：上海营养与健康研究所
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