miR-27a is highly expressed in H1650 cancer stem cells and regulates proliferation, migration, and invasion
文献类型：期刊论文
| 作者 | Luo, Wenwen1; Zhang, Deyi1; Ma, Shumin1; Wang, Chenyao1; Zhang, Qian1; Wang, Huafei1; Liu, Zhixue1; He, Kunyan2; , |
| 刊名 | JOURNAL OF CANCER RESEARCH AND THERAPEUTICS
 |
| 出版日期 | 2018 |
| 卷号 | 14期号:-页码:S1004-S1011 |
| 关键词 | Cancer stem cells epidermal growth factor receptor lung cancer microRNA |
| ISSN号 | 0973-1482 |
| DOI | 10.4103/0973-1482.199450 |
| 文献子类 | Article |
| 英文摘要 | Background: Cancer stem cells (CSCs) are responsible for tumor relapse after chemotherapy and radiotherapy in non-small cell lung cancer (NSCLC). The aim of this study is to explore the profile and role of microRNA (miRNA) in CSC of NSCLC. Materials and Methods: We studied the expression of stem cell marker in side population cells and serum-free cultured spheres of NSCLC. We identified that CD133(+) CD34 cells are NSCLC stem cell. We isolated CD133(+) CD34 cells and CD133 CD34(+) cells with MicroBead Kit. We verified that H1650 CD133(+) CD34 cells have CSC characteristics with doxorubicin, radiation, and xenograft. We studied miRNA expression profile in H1650 and HCC827 CD133(+) CD34 cells with microarray analysis. We detected proliferation, migration, and invasion with 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, scratch test, and Transwell chamber invasion assay, respectively. Results: CD133 and CD34 are CSC markers in H1650. We demonstrated that H1650 CD133(+) CD34 cells have CSC characteristics and found that miR-27a was highly expressed in H1650 CD133(+) CD34 cells. In addition, we showed that miR-27a regulates proliferation, migration, and invasion in H1650 cell line and demonstrated that miR-27a expression was positively related to epidermal growth factor receptor in NSCLC cell lines. Conclusions: CD133(+) CD34 is a CSC marker in H1650. miR-27a is highly expressed in H1650 CSCs and regulates cancer development in H1650. miR-27a may be a potential target for NSCLC therapy. |
| 学科主题 | Oncology |
| WOS关键词 | LUNG-CANCER ; SOLID TUMORS ; ADENOCARCINOMA ; RESISTANCE ; MUTATIONS ; MICRORNAS ; GEFITINIB ; SIGNATURE ; GENES |
| 语种 | 英语 |
| WOS记录号 | WOS:000453452300012 |
| 出版者 | WOLTERS KLUWER MEDKNOW PUBLICATIONS |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/577]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Univ Chinese Acad Sci, Chinese Acad Sci, Key Lab Nutr & Metab, Inst Nutr Sci,Shanghai Inst Biol Sci, Shanghai, Peoples R China; 2.Shanghai Jiao Tong Univ, Shanghai Ctr Syst Biomed, Key Lab Syst Biomed, Minist Educ, Shanghai, Peoples R China, |
| 推荐引用方式 GB/T 7714 | Luo, Wenwen,Zhang, Deyi,Ma, Shumin,et al. miR-27a is highly expressed in H1650 cancer stem cells and regulates proliferation, migration, and invasion[J]. JOURNAL OF CANCER RESEARCH AND THERAPEUTICS,2018,14(-):S1004-S1011. |
| APA | Luo, Wenwen.,Zhang, Deyi.,Ma, Shumin.,Wang, Chenyao.,Zhang, Qian.,...&,.(2018).miR-27a is highly expressed in H1650 cancer stem cells and regulates proliferation, migration, and invasion.JOURNAL OF CANCER RESEARCH AND THERAPEUTICS,14(-),S1004-S1011. |
| MLA | Luo, Wenwen,et al."miR-27a is highly expressed in H1650 cancer stem cells and regulates proliferation, migration, and invasion".JOURNAL OF CANCER RESEARCH AND THERAPEUTICS 14.-(2018):S1004-S1011. |
入库方式： OAI收割
来源：上海营养与健康研究所
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