Liver cancer: WISP3 suppresses hepatocellular carcinoma progression by negative regulation of beta-catenin/TCF/LEF signalling
文献类型：期刊论文
| 作者 | Gao, Hong2; Yin, Fen-Fen2; Guan, Dong-Xian2; Feng, Yu-Xiong2; Chen, Tian-Wei1,2; Jiang, Hao2; Zhang, Er-Bin1,2; Wang, Jing-Jing2; Ni, Qian-Zhi1,2; Yuan, Yan-Mei1,2 |
| 刊名 | CELL PROLIFERATION
 |
| 出版日期 | 2019 |
| 卷号 | 52期号:3页码:e12583 |
| ISSN号 | 0960-7722 |
| 关键词 | hepatocellular carcinoma metastasis tumorigenesis WISP3 beta-catenin |
| DOI | 10.1111/cpr.12583 |
| 文献子类 | Article |
| 英文摘要 | Objectives Wnt1-inducible signalling pathway protein 3 (WISP3/CCN6) belongs to the CCN (CYR61/CTGF/NOV) family of proteins, dysregulation of this family contributed to the tumorigenicity of various tumours. In this study, we need to explore its role in hepatocellular carcinoma that remains largely elusive. Materials and Methods The expression of WISP3/CCN6 was analysed by qRT-PCR and Western blotting. Effects of WISP3 on proliferation and metastasis of HCC cells were examined, respectively, by MTT assay and Boyden Chamber. Roles of WISP3 on HCC tumour growth and metastatic ability in vivo were detected in nude mice. Related mechanism study was confirmed by immunofluorescence and Western blotting. Results The expression of WISP3 was significantly downregulated in HCC clinical samples and cell lines, and reversely correlated with the tumour size. Forced expression of WISP3 in HCC cells significantly suppressed cell growth and migration in vitro as well as tumour growth and metastatic seeding in vivo. In contrast, downregulation of WISP3 accelerated cell proliferation and migration, and promoted in vivo metastasis. Further study revealed that WISP3 inhibited the translocation of beta-catenin to the nucleus by activating glycogen synthase kinase-3 beta (GSK3 beta). Moreover, constitutively active beta-catenin blocked the suppressive effects of WISP3 on HCC. Conclusions Our study showed that WISP3 suppressed the progression of HCC by negative regulation of beta-catenin/TCF/LEF signalling, providing WISP3 as a potential therapeutic candidate for HCC. |
| 学科主题 | Cell Biology |
| WOS关键词 | CCN FAMILY ; E-CADHERIN ; COLORECTAL CARCINOMAS ; WNT PATHWAY ; CATENIN ; PROLIFERATION ; EXPRESSION ; GROWTH ; CELLS ; GENE |
| 语种 | 英语 |
| 出版者 | WILEY |
| WOS记录号 | WOS:000469271700015 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/586]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Nutr & Hlth, Shanghai Inst Biol Sci, CAS Key Lab Nutr Metab & Food Safety, Shanghai, Peoples R China; 3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China; 4.Huzhou Univ, Peoples Hosp 1, Dept Surg, Huzhou, Peoples R China; 5.Southern Med Univ, Fengxian Hosp, Dept Gen Surg, Shanghai, Peoples R China; 6.Second Mil Med Univ, Eastern Hepatobiliary Surg Hosp, Shanghai, Peoples R China; 7.China Natl Ctr Food Safety Risk Assessment, NHC Key Lab Food Safety Risk Assessment, Beijing, Peoples R China, |
| 推荐引用方式 GB/T 7714 | Gao, Hong,Yin, Fen-Fen,Guan, Dong-Xian,et al. Liver cancer: WISP3 suppresses hepatocellular carcinoma progression by negative regulation of beta-catenin/TCF/LEF signalling[J]. CELL PROLIFERATION,2019,52(3):e12583. |
| APA | Gao, Hong.,Yin, Fen-Fen.,Guan, Dong-Xian.,Feng, Yu-Xiong.,Chen, Tian-Wei.,...&,.(2019).Liver cancer: WISP3 suppresses hepatocellular carcinoma progression by negative regulation of beta-catenin/TCF/LEF signalling.CELL PROLIFERATION,52(3),e12583. |
| MLA | Gao, Hong,et al."Liver cancer: WISP3 suppresses hepatocellular carcinoma progression by negative regulation of beta-catenin/TCF/LEF signalling".CELL PROLIFERATION 52.3(2019):e12583. |
入库方式： OAI收割
来源：上海营养与健康研究所
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