Proteomic profiling of HIV-1 infection of human CD4(+) T cells identifies PSGL-1 as an HIV restriction factor
文献类型：期刊论文
| 作者 | Liu, Ying6; Fu, Chunyan6; Zhang, Hang6; Li, Shuo6; Zhang, Tengjiang6; Gong, Jing6; Kong, Xiaohui6; Tan, Xu6; Fu, Yajing7,8; Zhou, Zheng8 |
| 刊名 | NATURE MICROBIOLOGY
 |
| 出版日期 | 2019 |
| 卷号 | 4期号:5页码:813-825 |
| ISSN号 | 2058-5276 |
| DOI | 10.1038/s41564-019-0372-2 |
| 文献子类 | Article |
| 英文摘要 | Human immunodeficiency virus (HIV) actively modulates the protein stability of host cells to optimize viral replication. To systematically examine this modulation in HIV infection, we used isobaric tag-based mass spectrometry to quantify changes in the abundance of over 14,000 proteins during HIV-1 infection of human primary CD4(+) T cells. We identified P-selectin glycoprotein ligand 1 (PSGL-1) as an HIV-1 restriction factor downregulated by HIV-1 Vpu, which binds to PSGL-1 and induces its ubiquitination and degradation through the ubiquitin ligase SCF beta-TrCP2. PSGL-1 is induced by interferon-gamma in activated CD4(+) T cells to inhibit HIV-1 reverse transcription and potently block viral infectivity by incorporating in progeny virions. This infectivity block is antagonized by Vpu via PSGL-1 degradation. We further show that PSGL-1 knockdown can significantly abolish the anti-HIV activity of interferon-gamma in primary CD4(+) T cells. Our study identifies an HIV restriction factor and a key mediator of interferon-gamma's anti-HIV activity. |
| 学科主题 | Microbiology |
| WOS关键词 | BETA-TRCP ; VPU ; DEGRADATION ; ANTAGONISM ; INDUCTION ; RELEASE ; SERINC5 ; PROTEIN ; SURFACE ; SAMHD1 |
| 语种 | 英语 |
| WOS记录号 | WOS:000465477000014 |
| 出版者 | NATURE PUBLISHING GROUP |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/587]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Chinese Acad Sci, Ctr Excellence Anim Evolut & Genet, Kunming, Yunnan, Peoples R China; 2.Capital Med Univ, Beijing Youan Hosp, Beijing, Peoples R China, 3.Chinese Acad Sci, Shanghai Inst Biol Sci, CAS MPG Partner Inst Computat Biol, Key Lab Computat Biol, Shanghai, Peoples R China; 4.Chinese Acad Sci, Inst Zool, Key Lab Zool Systemat & Evolut, Beijing, Peoples R China; 5.Zhejiang Univ, Affiliated Hosp 2, China Natl Minist Educ, Canc Inst,Key Lab Canc Prevent & Intervent,Sch Me, Hangzhou, Zhejiang, Peoples R China; 6.Tsinghua Univ, Tsinghua Peking Ctr Life Sci, MOE Key Lab Bioorgan Phosphorus Chem & Chem Biol, Sch Pharmaceut Sci,Sch Med,Ctr Infect Dis Res,Bei, Beijing, Peoples R China; 7.China Med Univ, Affiliated Hosp 1, Dept Lab Med, Key Lab AIDS Immunol Natl Hlth & Family Planning, Shenyang, Liaoning, Peoples R China; 8.George Mason Univ, Sch Syst Biol, Manassas, VA 20110 USA; 9.Fudan Univ, Zhongshan Hosp, Key Lab Carcinogenesis & Canc Invas, Liver Canc Inst,Minist Educ, Shanghai, Peoples R China; 10.Fudan Univ, Inst Biomed Sci, Shanghai, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Liu, Ying,Fu, Chunyan,Zhang, Hang,et al. Proteomic profiling of HIV-1 infection of human CD4(+) T cells identifies PSGL-1 as an HIV restriction factor[J]. NATURE MICROBIOLOGY,2019,4(5):813-825. |
| APA | Liu, Ying.,Fu, Chunyan.,Zhang, Hang.,Li, Shuo.,Zhang, Tengjiang.,...&,.(2019).Proteomic profiling of HIV-1 infection of human CD4(+) T cells identifies PSGL-1 as an HIV restriction factor.NATURE MICROBIOLOGY,4(5),813-825. |
| MLA | Liu, Ying,et al."Proteomic profiling of HIV-1 infection of human CD4(+) T cells identifies PSGL-1 as an HIV restriction factor".NATURE MICROBIOLOGY 4.5(2019):813-825. |
入库方式： OAI收割
来源：上海营养与健康研究所
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