Circular RNAs negatively regulate cancer stem cells by physically binding FMRP against CCAR1 complex in hepatocellular carcinoma
文献类型：期刊论文
| 作者 | Zhu, Yan-Jing1; Zheng, Bo1; Luo, Gui-Juan1; Lu, Xin-Yuan1; Lin, Xi-Meng1; Wu, Tong1; Li, Zhi-Xuan1; Wang, Hong-Yang1; Chen, Lei1,2; Ma, Xu-Kai7 |
| 刊名 | THERANOSTICS
 |
| 出版日期 | 2019 |
| 卷号 | 9期号:12页码:3526-3540 |
| ISSN号 | 1838-7640 |
| 关键词 | circular RNA hepatocellular carcinoma cancer stem cell RNA-binding protein |
| DOI | 10.7150/thno.32796 |
| 文献子类 | Article |
| 英文摘要 | Circular RNA (circRNA) possesses great pre-clinical diagnostic and therapeutic potentials in multiple cancers. It has been reported playing roles in multiple malignant behaviors including proliferation, migration, metastasis and chemoresistance. However, the underlying correlation between circRNAs and cancer stem cells (CSCs) has not been reported yet. Methods: circZKSCAN1 level was detected in HCC tissue microarrays to clarify its prognostic values. Gain and loss function experiments were applied to investigate the role of circZKSCAN1 in HCC stemness. Bioinformatic analysis was used to predict the possible downstream RNA binding protein and further RNA immunoprecipitation sequencing was carried out to identify the RBP-regulated genes. Results: The absence of circZKSCANI endowed several malignant properties including cancer stemness and tightly correlated with worse overall and recurrence-free survival rate in HCC. Bioinformatics analysis and RNA immunoprecipitation-sequencing (RIP-seq) results revealed that circZKSCAN1 exerted its inhibitive role by competitively binding FMRP, therefore, block the binding between FMRP and P-catenin-binding protein-cell cycle and apoptosis regulator 1 (CCAR1) mRNA, and subsequently restrain the transcriptional activity of Wnt signaling. In addition, RNA-splicing protein Quaking 5 was found downregulated in HCC tissues and responsible for the reduction of circZKSCAN1. Conclusion: Collectively, this study revealed the mechanisms underlying the regulatory role of circZKSCAN1 in HCC CSCs and identified the newly discovered Qki5-circZKSCAN1- FMRP-CCAR1-Wnt signaling axis as a potentially important therapeutic target for HCC treatment. |
| 学科主题 | Research & Experimental Medicine |
| WOS关键词 | GROWTH ; PROLIFERATION ; METASTASIS ; CYCLE ; IDENTIFICATION ; ACTIVATION ; PROTEIN-1 ; MIGRATION ; PROMOTES ; INVASION |
| 语种 | 英语 |
| 出版者 | IVYSPRING INT PUBL |
| WOS记录号 | WOS:000469953000010 |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/614]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Second Mil Med Univ, Eastern Hepatobiliary Surg Hosp, Int Cooperat Lab Signal Transduct, Shanghai 200438, Peoples R China; 2.Fudan Univ, Shanghai Canc Ctr, Dept Oncol, Shanghai Med Coll, Shanghai 200032, Peoples R China; 3.Cent S Univ, Dept Clin Pharmacol, Xiangya Hosp, Changsha, Hunan, Peoples R China; Second Mil Med Univ, Eastern Hepatobiliary Surg Hosp, Dept Oncol, North Moyu Rd 700, Shanghai, Peoples R China; 4.Fujian Med Univ, United Innovat Mengchao Hepatobiliary Technol Key, Mengchao Hepatobiliary Hosp, Fuzhou 350025, Fujian, Peoples R China; 5.Second Mil Med Univ, Dept Biliary Surg 1, Eastern Hepatobiliary Surg Hosp, Changhai Rd 225, Shanghai 200438, Peoples R China; 6.Shanghai Jiao Tong Univ, Sch Publ Hlth, Shanghai 200240, Peoples R China, 7.Chinese Acad Sci, Key Lab Computat Biol, CAS MPG Partner Inst Computat Biol, Univ Chinese Acad Sci,Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China; Second Mil Med Univ, Dept Pathol, Eastern Hepatobiliary Surg Hosp, Changhai Rd 225, Shanghai 200438, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Zhu, Yan-Jing,Zheng, Bo,Luo, Gui-Juan,et al. Circular RNAs negatively regulate cancer stem cells by physically binding FMRP against CCAR1 complex in hepatocellular carcinoma[J]. THERANOSTICS,2019,9(12):3526-3540. |
| APA | Zhu, Yan-Jing.,Zheng, Bo.,Luo, Gui-Juan.,Lu, Xin-Yuan.,Lin, Xi-Meng.,...&,.(2019).Circular RNAs negatively regulate cancer stem cells by physically binding FMRP against CCAR1 complex in hepatocellular carcinoma.THERANOSTICS,9(12),3526-3540. |
| MLA | Zhu, Yan-Jing,et al."Circular RNAs negatively regulate cancer stem cells by physically binding FMRP against CCAR1 complex in hepatocellular carcinoma".THERANOSTICS 9.12(2019):3526-3540. |
入库方式： OAI收割
来源：上海营养与健康研究所
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