Structural analysis of fungal CENP-H/I/K homologs reveals a conserved assembly mechanism underlying proper chromosome alignment
文献类型：期刊论文
| 作者 | Hu, Liqiao2; Huang, Hao2; Hei, Mohan2; Liu, Yunshan2; Wu, Mengying2; He, Xiaojing2; Tian, Wei2; Yang, Yang3; Li, Sheng4; Dou, Zhen5 |
| 刊名 | NUCLEIC ACIDS RESEARCH
 |
| 出版日期 | 2019 |
| 卷号 | 47期号:1页码:468-479 |
| ISSN号 | 0305-1048 |
| DOI | 10.1093/nar/gky1108 |
| 文献子类 | Article |
| 英文摘要 | The kinetochore is a proteinaceous complex that is essential for proper chromosome segregation. As a core member of the inner kinetochore, defects of each subunit in the CENP-H/I/K complex cause dysfunction of kinetochore that leads to chromosome mis-segregation and cell death. However, how the CENP-H/I/K complex assembles and promotes kinetochore function are poorly understood. We here determined the crystal structures of CENP-I N-terminus alone from Chaetomium thermophilum and its complex with CENP-H/K from Thielavia terrestris, and verified the identified interactions. The structures and biochemical analyses show that CENP-H and CENP-K form a heterodimer through both N- and C-terminal interactions. CENP-I integrates into the CENP-H/K complex by binding to the C-terminus of CENP-H, leading to formation of the ternary complex in which CENP-H is sandwiched between CENP-K and CENP-I. Our sequence comparisons and mutational analyses showed that this architecture of the CENP-H/I/K complex is conserved in human. Mutating the binding interfaces of CENP-H for either CENP-K or CENP-I significantly reduced their localizations at centromeres and induced massive chromosome alignment defects during mitosis, suggesting that the identified interactions are critical for CENP-H/I/K complex assembly at the centromere and kinetochore function. Altogether, our findings unveil the evolutionarily conserved assembly mechanism of the CENP-H/I/K complex that is critical for proper chromosome alignment. |
| 学科主题 | Biochemistry & Molecular Biology |
| WOS关键词 | CENTROMERIC NUCLEOSOME RECOGNITION ; OUTER KINETOCHORE ; A NUCLEOSOMES ; MOLECULAR-BASIS ; COMPLEX ; CHROMATIN ; RECRUITMENT ; COMPONENTS ; PLATFORM ; FORMS |
| 语种 | 英语 |
| WOS记录号 | WOS:000462586700040 |
| 出版者 | OXFORD UNIV PRESS |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/626]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Inst Biol Sci,CAS MPG Partner Inst Compu, Shanghai Inst Nutr & Hlth,CAS Key Lab Computat Bi, Shanghai 200031, Peoples R China; 2.Huazhong Univ Sci & Technol, Coll Life Sci & Technol, Minist Educ, Key Lab Mol Biophys, Wuhan 430074, Hubei, Peoples R China; 3.Univ Texas Southwestern Med Ctr Dallas, Dept Pharmacol, Dallas, TX 75390 USA; 4.ShanghaiTech Univ, Shanghai Inst Adv Immunochem Studies, Shanghai 201210, Peoples R China; 5.Univ Sci & Technol China, Hefei Natl Res Ctr Phys Sci Microscale, Anhui Key Lab Cellular Dynam & Chem Biol, Hefei 230027, Anhui, Peoples R China; 6.Tulane Univ, Hlth Sci Ctr, Dept Biochem & Mol Biol, New Orleans, LA 70112 USA; 7.Huazhong Univ Sci & Technol, Inst Brain Res, Collaborat Innovat Ctr Brain Sci, Wuhan 430030, Hubei, Peoples R China, |
| 推荐引用方式 GB/T 7714 | Hu, Liqiao,Huang, Hao,Hei, Mohan,et al. Structural analysis of fungal CENP-H/I/K homologs reveals a conserved assembly mechanism underlying proper chromosome alignment[J]. NUCLEIC ACIDS RESEARCH,2019,47(1):468-479. |
| APA | Hu, Liqiao.,Huang, Hao.,Hei, Mohan.,Liu, Yunshan.,Wu, Mengying.,...&,.(2019).Structural analysis of fungal CENP-H/I/K homologs reveals a conserved assembly mechanism underlying proper chromosome alignment.NUCLEIC ACIDS RESEARCH,47(1),468-479. |
| MLA | Hu, Liqiao,et al."Structural analysis of fungal CENP-H/I/K homologs reveals a conserved assembly mechanism underlying proper chromosome alignment".NUCLEIC ACIDS RESEARCH 47.1(2019):468-479. |
入库方式： OAI收割
来源：上海营养与健康研究所
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