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Accelerating precision medicine through genetic and genomic big data analysis

文献类型：期刊论文

作者Cai, Yudong1; Huang, Tao2
刊名BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE
出版日期2018
卷号1864期号:6页码:2215-2217
关键词Base editing Human cleaving embryos Homozygotic nucleotide substitution
ISSN号0925-4439
DOI10.1016/j.bbadis.2018.03.012
文献子类Editorial Material
英文摘要Base editing installs a precise nucleotide change in specific gene loci without causing a double-strand break. Its efficiency in human embryos is generally low, limiting its utility in functional genetic studies. Here, we report that injecting base editors into human cleaving two-cell and four-cell embryos results in much higher (up to 13-fold) homozygotic nucleotide substitution efficiency as opposed to MII oocytes or zygotes. Furthermore, as a proof-of-principle study, a point mutation can be efficiently corrected by our method. Our study indicates that human cleaving embryos provide an efficient base editing window for robust gene disruption and correction.
学科主题Biochemistry & Molecular Biology ; Biophysics ; Cell Biology
语种英语
WOS记录号WOS:000432105500001
出版者ELSEVIER SCIENCE BV
版本出版稿
源URL[http://202.127.25.144/handle/331004/632]  
专题中国科学院上海生命科学研究院营养科学研究所
作者单位1.Shanghai Univ, Sch Life Sci, Shanghai 200444, Peoples R China;
2.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Hlth Sci, Shanghai 200031, Peoples R China,
推荐引用方式
GB/T 7714		 Cai, Yudong,Huang, Tao. Accelerating precision medicine through genetic and genomic big data analysis[J]. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE,2018,1864(6):2215-2217.
APA Cai, Yudong,&Huang, Tao.(2018).Accelerating precision medicine through genetic and genomic big data analysis.BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE,1864(6),2215-2217.
MLA Cai, Yudong,et al."Accelerating precision medicine through genetic and genomic big data analysis".BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE 1864.6(2018):2215-2217.

入库方式： OAI收割

来源：上海营养与健康研究所

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