APOBEC3 induces mutations during repair of CRISPR-Cas9-generated DNA breaks
文献类型：期刊论文
| 作者 | Lei, Liqun3,4,5; Hu, Bian3; Wang, Lijie3,4,5; Shang, Wanjing3,4,5; Zhuang, Min3; Huang, Xingxu3; Chen, Jia3; Yan, Lei1,4,5; Chen, Hongquan6,7; Cui, Yiqiang6 |
| 刊名 | NATURE STRUCTURAL & MOLECULAR BIOLOGY
 |
| 出版日期 | 2018 |
| 卷号 | 25期号:1页码:45-+ |
| ISSN号 | 1545-9993 |
| DOI | 10.1038/s41594-017-0004-6 |
| 文献子类 | Article |
| 英文摘要 | The APOBEC-AID family of cytidine deaminase prefers single-stranded nucleic acids for cytidine-to-uridine deamination. Single-stranded nucleic acids are commonly involved in the DNA repair system for breaks generated by CRISPR-Cas9. Here, we show in human cells that APOBEC3 can trigger cytidine deamination of single-stranded oligodeoxynucleotides, which ultimately results in base substitution mutations in genomic DNA through homology-directed repair (HDR) of Cas9-generated double-strand breaks. In addition, the APOBEC3-catalyzed deamination in genomic single-stranded DNA formed during the repair of Cas9 nickase-generated single-strand breaks in human cells can be further processed to yield mutations mainly involving insertions or deletions (indels). Both APOBEC3-mediated deamination and DNA-repair proteins play important roles in the generation of these indels. Therefore, optimizing conditions for the repair of CRISPR-Cas9-generated DNA breaks, such as using double-stranded donors in HDR or temporarily suppressing endogenous APOBEC3s, can repress these unwanted mutations in genomic DNA. |
| 学科主题 | Biochemistry & Molecular Biology ; Biophysics ; Cell Biology |
| WOS关键词 | MODIFIED CRISPR/CAS9 SYSTEM ; HUMAN CANCERS ; RETROVIRAL RESTRICTION ; MUSCULAR-DYSTROPHY ; EUKARYOTIC GENOMES ; DEAMINASE FUSION ; END RESECTION ; TARGET BASE ; MOUSE MODEL ; RICE |
| 语种 | 英语 |
| WOS记录号 | WOS:000423547700008 |
| 出版者 | NATURE PUBLISHING GROUP |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/633]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.ShanghaiTech Univ, Shanghai Inst Adv Immunochem Studies, Shanghai, Peoples R China; 2.Nanjing Univ, Natl Resource Ctr Mutant Mice, Model Anim Res Ctr, MOE Key Lab Model Anim Dis Study, Nanjing, Jiangsu, Peoples R China, 3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China; 4.Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, Shanghai, Peoples R China; 5.Univ Chinese Acad Sci, Beijing, Peoples R China; 6.Nanjing Med Univ, Dept Histol & Embryol, State Key Lab Reprod Med, Nanjing, Jiangsu, Peoples R China; 7.Wenzhou Med Univ, Sch Lab Med & Life Sci, Wenzhou, Peoples R China; 8.Chinese Acad Sci, Shanghai Inst Biol Sci, CAS MPG Partner Inst Computat Biol, Key Lab Computat Biol, Shanghai, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Lei, Liqun,Hu, Bian,Wang, Lijie,et al. APOBEC3 induces mutations during repair of CRISPR-Cas9-generated DNA breaks[J]. NATURE STRUCTURAL & MOLECULAR BIOLOGY,2018,25(1):45-+. |
| APA | Lei, Liqun.,Hu, Bian.,Wang, Lijie.,Shang, Wanjing.,Zhuang, Min.,...&,.(2018).APOBEC3 induces mutations during repair of CRISPR-Cas9-generated DNA breaks.NATURE STRUCTURAL & MOLECULAR BIOLOGY,25(1),45-+. |
| MLA | Lei, Liqun,et al."APOBEC3 induces mutations during repair of CRISPR-Cas9-generated DNA breaks".NATURE STRUCTURAL & MOLECULAR BIOLOGY 25.1(2018):45-+. |
入库方式： OAI收割
来源：上海营养与健康研究所
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