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Base editing with a Cpf1-cytidine deaminase fusion

文献类型：期刊论文

作者Li, Xiaosa1,3,4; Liu, Yajing1,3,4; Wang, Xiao1,3,4; Lu, Zongyang1,3,4; Zhang, Yuxi3; Wu, Jing3; Huang, Xingxu3; Chen, Jia3; Wang, Ying5; Wei, Jia5
刊名NATURE BIOTECHNOLOGY
出版日期2018
卷号36期号:4页码:324-+
关键词hepatocellular carcinoma metastasis tumorigenesis WISP3 beta-catenin
ISSN号1087-0156
DOI10.1038/nbt.4102
文献子类Article
英文摘要The targeting range of CRISPR-Cas9 base editors (BEs) is limited by their G/C-rich protospacer-adjacent motif (PAM) sequences. To overcome this limitation, we developed a CRISPR-Cpf1-based BE by fusing the rat cytosine deaminase APOBEC1 to a catalytically inactive version of Lachnospiraceae bacterium Cpf1. The base editor recognizes a T-rich PAM sequence and catalyzes C-to-T conversion in human cells, while inducing low levels of indels, non-C-to-T substitutions and off-target editing.
学科主题Biotechnology & Applied Microbiology
WOS关键词ALTERED PAM SPECIFICITIES ; DNA CLEAVAGE ; HUMAN-CELLS ; CPF1 ; ENDONUCLEASES ; NUCLEASES ; VERSATILE
语种英语
WOS记录号WOS:000429342400016
出版者NATURE PUBLISHING GROUP
版本出版稿
源URL[http://202.127.25.144/handle/331004/642]  
专题中国科学院上海生命科学研究院营养科学研究所
作者单位1.Univ Chinese Acad Sci, Beijing, Peoples R China;
2.Shanghai Tech Univ, Shanghai Inst Adv Immunochem Studies, Shanghai, Peoples R China,
3.ShanghaiTech Univ, Sch Life Sci & Technol, Shanghai, Peoples R China;
4.Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, Shanghai, Peoples R China;
5.Chinese Acad Sci, Univ Chinese Acad Sci, Shanghai Inst Biol Sci, CAS MPG Partner Inst Computat Biol,Key Lab Comput, Shanghai, Peoples R China;
推荐引用方式
GB/T 7714		 Li, Xiaosa,Liu, Yajing,Wang, Xiao,et al. Base editing with a Cpf1-cytidine deaminase fusion[J]. NATURE BIOTECHNOLOGY,2018,36(4):324-+.
APA Li, Xiaosa.,Liu, Yajing.,Wang, Xiao.,Lu, Zongyang.,Zhang, Yuxi.,...&,.(2018).Base editing with a Cpf1-cytidine deaminase fusion.NATURE BIOTECHNOLOGY,36(4),324-+.
MLA Li, Xiaosa,et al."Base editing with a Cpf1-cytidine deaminase fusion".NATURE BIOTECHNOLOGY 36.4(2018):324-+.

入库方式： OAI收割

来源：上海营养与健康研究所

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