Degree of endplate chondrocyte degeneration in different tension regions during mechanical stimulation
文献类型：期刊论文
| 作者 | Xu, Yongming1; Gao, Zhi1; Xiao, Liang1; Xu, Hongguang1; Xu, Shujuan4; Xiao, Fei5; Zhang, Xiaoling5; Zhang, Xiaoling2,3; , |
| 刊名 | MOLECULAR MEDICINE REPORTS
 |
| 出版日期 | 2018 |
| 卷号 | 17期号:3页码:4415-4421 |
| 关键词 | FX-5000T tension system endplate chondrocytes intermittent cyclic mechanical tension chondrocyte degeneration cell morphology |
| ISSN号 | 1791-2997 |
| DOI | 10.3892/mmr.2018.8435 |
| 文献子类 | Article |
| 英文摘要 | The aim of this study was to explore the degree of degeneration of endplate chondrocytes in different tension regions induced by intermittent cyclic mechanical tension (ICMT) in vitro. Rat endplate chondrocytes were harvested and treated with 10% ICMT for 8 h/day with a frequency of 0.5 Hz. A cartilage degeneration model was induced using an FX-5000T cell strain-loading system. The experiment was divided into the central region and the peripheral region, according to the contact area between the loading post and the six-well flexible silicone rubber BioFlex plates. Toluidine blue and phalloidin staining were used to observe the morphological changes of cells following mechanical stimulation. Apoptosis was detected by flow cytometry and the mRNA and protein expression levels of collagen type II 1, aggrecan, SRY-box 9 and matrix metalloproteinase 13 were detected by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blotting, respectively. Endplate chondrocytes exhibited degenerative alterations under mechanical conditions of 10% ICMT and 0.5 Hz at 8 h/day. Toluidine blue and phalloidin staining demonstrated that the cells in the peripheral region were more slender compared with cells in the central region, but RT-qPCR and western blotting results demonstrated that the degree of cell degeneration between the two groups was not statistically differences. So that cell morphological alteration does not imply that cells have undergone degeneration. |
| 学科主题 | Oncology ; Research & Experimental Medicine |
| WOS关键词 | IN-VITRO ; EXPRESSION ; BIOLOGY ; STRAIN ; CELLS ; SHAPE |
| 语种 | 英语 |
| WOS记录号 | WOS:000424400000125 |
| 出版者 | SPANDIDOS PUBL LTD |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/650]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Wannan Med Coll, Yijishan Hosp, Affiliated Hosp 1, Dept Orthoped Surg, 2 Zheshan West Rd, Wuhu 241001, Anhui, Peoples R China; 2.Chinese Acad Sci, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China, 3.Shanghai Jiao Tong Univ, Sch Med, Inst Hlth Sci, Key Lab Stem Cell Biol, Shanghai 200031, Peoples R China; 4.Wannan Med Coll, Yijishan Hosp, Affiliated Hosp 1, Dept Surg, Wuhu 241001, Anhui, Peoples R China; 5.Shanghai Jiao Tong Univ, Sch Med, Xinhua Hosp, Dept Orthoped Surg, 1665 Kongjiang Rd, Shanghai 200011, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Xu, Yongming,Gao, Zhi,Xiao, Liang,et al. Degree of endplate chondrocyte degeneration in different tension regions during mechanical stimulation[J]. MOLECULAR MEDICINE REPORTS,2018,17(3):4415-4421. |
| APA | Xu, Yongming.,Gao, Zhi.,Xiao, Liang.,Xu, Hongguang.,Xu, Shujuan.,...&,.(2018).Degree of endplate chondrocyte degeneration in different tension regions during mechanical stimulation.MOLECULAR MEDICINE REPORTS,17(3),4415-4421. |
| MLA | Xu, Yongming,et al."Degree of endplate chondrocyte degeneration in different tension regions during mechanical stimulation".MOLECULAR MEDICINE REPORTS 17.3(2018):4415-4421. |
入库方式： OAI收割
来源：上海营养与健康研究所
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