Single-cell RNA-Seq analysis reveals dynamic trajectories during mouse liver development
文献类型：期刊论文
| 作者 | Su, Xianbin3,4; Shi, Yi3,4; Zou, Xin3,4; Lu, Zhao-Ning3,4; Wu, Chong-Chao3,4; Cui, Xiao-Fang3,4; He, Kun-Yan3,4; Luo, Qing3,4; Qu, Yu-Lan3,4; Wang, Na3,4 |
| 刊名 | BMC GENOMICS
 |
| 出版日期 | 2017 |
| 卷号 | 18期号:-页码:946 |
| 关键词 | Liver stem/progenitor cells Single-cell RNA-Seq Developmental trajectory Cholangiocyte Fate decision |
| ISSN号 | 1471-2164 |
| DOI | 10.1186/s12864-017-4342-x |
| 文献子类 | Article |
| 英文摘要 | Background: The differentiation and maturation trajectories of fetal liver stem/progenitor cells (LSPCs) are not fully understood at single-cell resolution, and a priori knowledge of limited biomarkers could restrict trajectory tracking. Results: We employed marker-free single-cell RNA-Seq to characterize comprehensive transcriptional profiles of 507 cells randomly selected from seven stages between embryonic day 11.5 and postnatal day 2.5 during mouse liver development, and also 52 Epcam-positive cholangiocytes from postnatal day 3.25 mouse livers. LSPCs in developing mouse livers were identified via marker-free transcriptomic profiling. Single-cell resolution dynamic developmental trajectories of LSPCs exhibited contiguous but discrete genetic control through transcription factors and signaling pathways. The gene expression profiles of cholangiocytes were more close to that of embryonic day 11.5 rather than other later staged LSPCs, cuing the fate decision stage of LSPCs. Our marker-free approach also allows systematic assessment and prediction of isolation biomarkers for LSPCs. Conclusions: Our data provide not only a valuable resource but also novel insights into the fate decision and transcriptional control of self-renewal, differentiation and maturation of LSPCs. |
| 学科主题 | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| WOS关键词 | STEM-CELLS ; STEM/PROGENITOR CELLS ; SONIC-HEDGEHOG ; EXPRESSION ; DIFFERENTIATION ; ORGANOGENESIS ; HEPATOBLASTS ; HEPATOCYTES ; ENRICHMENT ; PATHWAY |
| 语种 | 英语 |
| WOS记录号 | WOS:000416967200006 |
| 出版者 | BIOMED CENTRAL LTD |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/656]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.CAS MPG Partner Inst Computat Biol, Key Lab Computat Biol, 320 Yueyang Rd, Shanghai, Peoples R China; 2.Chinese Natl Human Genome Ctr, Shanghai MOST Key Lab Dis & Hlth Genom, Shanghai, Shanghai, Peoples R China, 3.Shanghai Jiao Tong Univ, Shanghai Ctr Syst Biomed, Minist Educ, Key Lab Syst Biomed, 800 Dongchuan Rd, Shanghai 200240, Peoples R China; 4.Shanghai Jiao Tong Univ, Shanghai Ctr Syst Biomed, Collaborat Innovat Ctr Syst Biomed, 800 Dongchuan Rd, Shanghai 200240, Peoples R China; 5.Univ Sydney, Sch Math & Stat, Sydney, NSW, Australia; |
| 推荐引用方式 GB/T 7714 | Su, Xianbin,Shi, Yi,Zou, Xin,et al. Single-cell RNA-Seq analysis reveals dynamic trajectories during mouse liver development[J]. BMC GENOMICS,2017,18(-):946. |
| APA | Su, Xianbin.,Shi, Yi.,Zou, Xin.,Lu, Zhao-Ning.,Wu, Chong-Chao.,...&,.(2017).Single-cell RNA-Seq analysis reveals dynamic trajectories during mouse liver development.BMC GENOMICS,18(-),946. |
| MLA | Su, Xianbin,et al."Single-cell RNA-Seq analysis reveals dynamic trajectories during mouse liver development".BMC GENOMICS 18.-(2017):946. |
入库方式： OAI收割
来源：上海营养与健康研究所
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