Identification and characterization of functional modules reflecting transcriptome transition during human neuron maturation
文献类型：期刊论文
| 作者 | He, Zhisong1,2,3; Yu, Qianhui3; Yu, Qianhui4; , |
| 刊名 | BMC GENOMICS
 |
| 出版日期 | 2018 |
| 卷号 | 19期号:-页码:262 |
| 关键词 | Neuron maturation Single-cell RNA-seq Protein-protein interaction network |
| ISSN号 | 1471-2164 |
| DOI | 10.1186/s12864-018-4649-2 |
| 文献子类 | Article |
| 英文摘要 | Background: Neuron maturation is a critical process in neurogenesis, during which neurons gain their morphological, electrophysiological and molecular characteristics for their functions as the central components of the nervous system. Results: To better understand the molecular changes during this process, we combined the protein-protein interaction network and public single cell RNA-seq data of mature and immature neurons to identify functional modules relevant to the neuron maturation process in humans. Among the 109 functional modules in total, 33 showed significant gene expression level changes (discriminating modules) which participate in varied functions including energy consumption, synaptic functions and housekeeping functions such as translation and splicing. Based on the identified modules, we trained a neuron maturity index (NMI) model for the quantification of maturation states of single neurons or purified bulk neurons. Applied to multiple single neuron transcriptome data sets of neuron development in humans and mice, the NMI model made estimation of neuron maturity states which were significantly correlated with the neuron maturation trajectories in both species, implying the reproducibility and conservation of the identified transcriptome transition. Conclusion: We identified 33 discriminating modules whose activities were significantly correlated with single neuron maturity states, which may play important roles in the neuron maturation process. |
| 学科主题 | Biotechnology & Applied Microbiology ; Genetics & Heredity |
| WOS关键词 | REACTOME PATHWAY KNOWLEDGEBASE ; STEM-CELLS ; BINDING PROTEIN ; HUMAN BRAIN ; RNA-SEQ ; DIFFERENTIATION ; METABOLISM ; REGULATORS ; DIVERSITY ; DYNAMICS |
| 语种 | 英语 |
| WOS记录号 | WOS:000430805700002 |
| 出版者 | BIOMED CENTRAL LTD |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/675]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Skolkovo Inst Sci & Technol, Ctr Data Intens Biomed & Biotechnol, Moscow 143028, Russia; 2.Max Planck Inst Evolutionary Anthropol, Dept Evolutionary Genet, Deutsch Pl 6, D-04103 Leipzig, Germany, 3.Chinese Acad Sci, CAS Key Lab Computat Biol, CAS MPG Partner Inst Computat Biol PICB, SIBS, Shanghai 200031, Peoples R China; 4.Chinese Acad Sci, Shanghai Inst Biol Sci, Univ Chinese Acad Sci, Shanghai 200031, Peoples R China; |
| 推荐引用方式 GB/T 7714 | He, Zhisong,Yu, Qianhui,Yu, Qianhui,et al. Identification and characterization of functional modules reflecting transcriptome transition during human neuron maturation[J]. BMC GENOMICS,2018,19(-):262. |
| APA | He, Zhisong,Yu, Qianhui,Yu, Qianhui,&,.(2018).Identification and characterization of functional modules reflecting transcriptome transition during human neuron maturation.BMC GENOMICS,19(-),262. |
| MLA | He, Zhisong,et al."Identification and characterization of functional modules reflecting transcriptome transition during human neuron maturation".BMC GENOMICS 19.-(2018):262. |
入库方式： OAI收割
来源：上海营养与健康研究所
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