MRPL33 and its splicing regulator hnRNPK are required for mitochondria function and implicated in tumor progression
文献类型：期刊论文
| 作者 | Liu, L.3; Luo, C.3; Luo, Y.3; Chen, L.3; Liu, Y.3; Wang, Y.3; Zhang, Y.3; Wei, N.3; Xie, Z.3; Feng, Y.3 |
| 刊名 | ONCOGENE
 |
| 出版日期 | 2018 |
| 卷号 | 37期号:1页码:86-94 |
| 关键词 | Embryo development single cell expression pattern rule multi-class classification |
| ISSN号 | 0950-9232 |
| DOI | 10.1038/onc.2017.314 |
| 文献子类 | Article |
| 英文摘要 | MRPL33 gene encodes a large mitoribosomal subunit protein, which may be involved in mitochondrial translation. Although two splice variants of MRPL33 have been described, its splicing regulation remains elusive. Here we observed that inclusion of alternative exon 3 was greatly promoted in a panel of human cancer cells. Depletion of the exon 3-containing long isoform of MRPL33 (MRPL33-L) led to impaired proliferation and increased apoptosis in cancer cell lines and in a xenograft model. MRPL33-L knockdown could also induce mitochondrial dysfunction including increased accumulation of reactive oxygen species, decreased ATP production and 16 S rRNA levels. We further showed that alternative splicing of MRPL33-L pre-mRNA is regulated by hnRNPK and that knocking down hnRNPK could phenocopy MRPL33-L depletion. More importantly, overexpression of MRPL33-L could increase tumorigenic potential of hnRNPK-depleted cancer cells, likely indicating that hnRNPK mediates tumorigenesis through splicing regulation of MRPL33 pre-mRNA. Finally, we found that inclusion of MRPL33 exon 3 was promoted in human colorectal cancer tissues and this was correlated with hnRNPK levels. In summary, our findings underscore the biological significance of MRPL33-L and hnRNPK in the tumor formation and identifies hnRNPK as a critical splicing regulator of MRPL33 pre-mRNA in cancer cells. |
| 学科主题 | Biochemistry & Molecular Biology ; Oncology ; Cell Biology ; Genetics & Heredity |
| WOS关键词 | NUCLEAR RIBONUCLEOPROTEIN-K ; MT-CYB ; CANCER ; REVEALS ; PROTEIN ; EVENTS ; GENES ; MECHANISMS ; FAILURE ; ROLES |
| 语种 | 英语 |
| WOS记录号 | WOS:000422625000009 |
| 出版者 | NATURE PUBLISHING GROUP |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/684]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Fudan Univ, Zhongshan Hosp, Sch Med, Dept Surg, Shanghai, Peoples R China; 2.Zhejiang Agr & Forestry Univ, Nurturing Stn, State Key Lab Subtrop Silviculture, Linan, Peoples R China, 3.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Inst Biol Sci, Key Lab Food Safety Res,Inst Nutr Sci, Shanghai, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Liu, L.,Luo, C.,Luo, Y.,et al. MRPL33 and its splicing regulator hnRNPK are required for mitochondria function and implicated in tumor progression[J]. ONCOGENE,2018,37(1):86-94. |
| APA | Liu, L..,Luo, C..,Luo, Y..,Chen, L..,Liu, Y..,...&,.(2018).MRPL33 and its splicing regulator hnRNPK are required for mitochondria function and implicated in tumor progression.ONCOGENE,37(1),86-94. |
| MLA | Liu, L.,et al."MRPL33 and its splicing regulator hnRNPK are required for mitochondria function and implicated in tumor progression".ONCOGENE 37.1(2018):86-94. |
入库方式： OAI收割
来源：上海营养与健康研究所
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