Laminar Flow Attenuates Macrophage Migration Inhibitory Factor Expression in Endothelial Cells
文献类型：期刊论文
| 作者 | Qiao, Congzhen3,4; Chen, Y. Eugene3,7; Li, Shengdi5,6; Lu, Haocheng7; Fan, Yanbo7; Guo, Yanhong7; Zhang, Jifeng7; Meng, Fan1,2; , |
| 刊名 | SCIENTIFIC REPORTS
 |
| 出版日期 | 2018 |
| 卷号 | 8期号:-页码:2360 |
| ISSN号 | 2045-2322 |
| DOI | 10.1038/s41598-018-20885-1 |
| 文献子类 | Article |
| 英文摘要 | Macrophage migration inhibitory factor (MIF) is a non-canonical cytokine that is involved in multiple inflammatory diseases, including atherosclerosis. High MIF expression found in leukocytes which facilitates the initiation and progression of atherosclerosis. However, little is known about biomechanical forces in the induction of MIF in endothelial cells (ECs). Here, we show that laminar shear stress (LS) inhibits the expression of MIF in ECs. By profiling the whole transcriptome of human coronary artery ECs under different shear stress, we found that athero-protective LS attenuates the expression of MIF whereas pro-atherosclerotic oscillatory shear stress (OS) significantly increased the expression of MIF. En face staining of rabbit aorta revealed high MIF immunoreactivity in lesser curvature as well as arterial bifurcation areas where OS is predominant. Mechanistically, we found that Krupple like factor 2 (KLF2) is required for inhibition of MIF expression in ECs in the context of shear stress. Knockdown of KLF2 abolishes LS-dependent MIF inhibition while overexpression of KLF2 significantly attenuated MIF expression. Overall, the present work showed that MIF is a shear stress-sensitive cytokine and is transcriptionally regulated by KLF2, suggesting that LS exerts its athero-protective effect in part by directly inhibiting pro-inflammatory MIF expression. |
| 学科主题 | Science & Technology - Other Topics |
| WOS关键词 | APOLIPOPROTEIN E-DEFICIENT ; WALL SHEAR-STRESS ; ATHEROSCLEROTIC PLAQUES ; INFLAMMATORY RESPONSE ; ADHESION MOLECULES ; DISTURBED FLOW ; FACTOR MIF ; IN-VIVO ; ASSOCIATION ; ATHEROGENESIS |
| 语种 | 英语 |
| WOS记录号 | WOS:000424087700032 |
| 出版者 | NATURE PUBLISHING GROUP |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/689]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Univ Michigan, Med Ctr, Dept Psychiat, Ann Arbor, MI 48109 USA; 2.Univ Michigan, Med Ctr, Behav Neurosci Inst, 1500 E Med Ctr Dr, Ann Arbor, MI 48109 USA, 3.Univ Michigan, Med Ctr, Dept Pharmacol, Ann Arbor, MI 48109 USA; 4.Shanghai Jiao Tong Univ, Sch Med, Shanghai 200025, Peoples R China; 5.Chinese Acad Sci, CAS MPG Partner Inst Computat Biol, Key Lab Computat Biol, Shanghai Inst Biol Sci, Shanghai 200031, Peoples R China; 6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China; 7.Univ Michigan, Med Ctr, Dept Internal Med, Frankel Cardiovasc Ctr, Ann Arbor, MI 48109 USA; |
| 推荐引用方式 GB/T 7714 | Qiao, Congzhen,Chen, Y. Eugene,Li, Shengdi,et al. Laminar Flow Attenuates Macrophage Migration Inhibitory Factor Expression in Endothelial Cells[J]. SCIENTIFIC REPORTS,2018,8(-):2360. |
| APA | Qiao, Congzhen.,Chen, Y. Eugene.,Li, Shengdi.,Lu, Haocheng.,Fan, Yanbo.,...&,.(2018).Laminar Flow Attenuates Macrophage Migration Inhibitory Factor Expression in Endothelial Cells.SCIENTIFIC REPORTS,8(-),2360. |
| MLA | Qiao, Congzhen,et al."Laminar Flow Attenuates Macrophage Migration Inhibitory Factor Expression in Endothelial Cells".SCIENTIFIC REPORTS 8.-(2018):2360. |
入库方式： OAI收割
来源：上海营养与健康研究所
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