LncRNA OIP5-AS1 regulates radioresistance by targeting DYRK1A through miR-369-3p in colorectal cancer cells
文献类型：期刊论文
| 作者 | Zou, Yanmei2; Yao, Shuo2; Chen, Xiuqiong2; Liu, Dian2; Wang, Jianhua2; Yuan, Xun2; Rao, Jie2; Xiong, Huihua2; Yu, Shiying2; Yuan, Xianglin2 |
| 刊名 | EUROPEAN JOURNAL OF CELL BIOLOGY
 |
| 出版日期 | 2018 |
| 卷号 | 97期号:5页码:369-378 |
| 关键词 | Colorectal cancer OIP5-AS1 miR-369-3p DYRK1A |
| ISSN号 | 0171-9335 |
| DOI | 10.1016/j.ejcb.2018.04.005 |
| 文献子类 | Article |
| 英文摘要 | Object This study aimed to investigate the role of lncRNA OIP5-AS1 in regulating radioresistance of colorectal cancer (CRC) cells. Methods: Microarray analysis was used to screen out IncRNAs differentially expressed in radio-resistant CRC cell lines. Expression levels of OIP5-AS1, miR-369-3p and DYRKIA in CRC cell lines were measured by qRT-PCR. Protein expression of DYRKIA was determined by western blot. The target relationships among OIP5-AS1, miR-369-3p and DYRKIA were validated by dual luciferase reporter assay. Impacts of OIP5-AS1 or DYRKIA on CRC cellular activity and apoptosis were investigated by MIT assay, clonogenic survival assay and flow cytometry to analyze OIP5-AS1 or DYRK1A's effect on radioresistance of CRC cells. Results: LncRNA OIP5-AS1 and DYRKIA were down-regulated in radio-resistant CRC cell lines. OIP5-AS1 suppressed the expression of miR-369-3p, thus up-regulating DYRKIA, the downstream gene of miR-369-3p. OIP5AS1 and DYRKIA impaired cell clonogenic survival and promoted cell apoptosis after irradiation, improving radiosensitivity of CRC cells. Conclusion: LncRNA OIP5-AS1 suppressed cell viability, promoted radio-induced apoptosis, and enhanced the radiosensitivity of CRC cells by regulating DYRKIA expression through miR-369-3p. |
| 学科主题 | Cell Biology |
| WOS关键词 | SIGNALING PATHWAY ; DOWN-REGULATION ; NONCODING RNAS ; EXPRESSION ; RADIOTHERAPY ; MIGRATION ; MIR-1246 ; BINDING |
| 语种 | 英语 |
| WOS记录号 | WOS:000440261600005 |
| 出版者 | ELSEVIER GMBH, URBAN & FISCHER VERLAG |
| 版本 | 出版稿 |
| 源URL | [http://202.127.25.144/handle/331004/690]  |
| 专题 | 中国科学院上海生命科学研究院营养科学研究所 |
| 作者单位 | 1.Shanghai Inst Biol, Inst Hlth Sci, Key Lab Stem Cell Biol, Shanghai 200031, Peoples R China; 2.Huazhong Univ Sci & Technol, Tongji Med Coll, Tongji Hosp, Dept Oncol, 1095 Jiefang Ave, Wuhan 430030, Hubei, Peoples R China; 3.Univ Southampton, Fac Nat & Environm Sci, Biol Sci, Southampton SO17 1BJ, Hants, England, 4.Huazhong Univ Sci & Technol, Sch Basic Med, Dept Biochem & Mol Biol, Wuhan 430030, Hubei, Peoples R China; |
| 推荐引用方式 GB/T 7714 | Zou, Yanmei,Yao, Shuo,Chen, Xiuqiong,et al. LncRNA OIP5-AS1 regulates radioresistance by targeting DYRK1A through miR-369-3p in colorectal cancer cells[J]. EUROPEAN JOURNAL OF CELL BIOLOGY,2018,97(5):369-378. |
| APA | Zou, Yanmei.,Yao, Shuo.,Chen, Xiuqiong.,Liu, Dian.,Wang, Jianhua.,...&,.(2018).LncRNA OIP5-AS1 regulates radioresistance by targeting DYRK1A through miR-369-3p in colorectal cancer cells.EUROPEAN JOURNAL OF CELL BIOLOGY,97(5),369-378. |
| MLA | Zou, Yanmei,et al."LncRNA OIP5-AS1 regulates radioresistance by targeting DYRK1A through miR-369-3p in colorectal cancer cells".EUROPEAN JOURNAL OF CELL BIOLOGY 97.5(2018):369-378. |
入库方式： OAI收割
来源：上海营养与健康研究所
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