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Suppressing Nodal Signaling Activity Predisposes Ectodermal Differentiation of Epiblast Stem Cells

文献类型：期刊论文

作者Liu, Chang3; Wang, Ran3; Yang, Xianfa3; Chen, Jun3; Cui, Guizhong3; Guo, Wenke3,6; Chen, Yingying3,6; Peng, Guangdun3; Jing, Naihe3,6; He, Zhisong4
刊名STEM CELL REPORTS
出版日期2018
卷号11期号:1页码:43-57
关键词PI3K Peptide Therapy Gastric cancers Mouse model
ISSN号2213-6711
DOI10.1016/j.stemcr.2018.05.019
文献子类Article
英文摘要The molecular mechanism underpinning the specification of the ectoderm, a transient germ-layer tissue, during mouse gastrulation was examined here in a stem cell-based model. We captured a self-renewing cell population with enhanced ectoderm potency from mouse epiblast stem cells (EpiSCs) by suppressing Nodal signaling activity. The transcriptome of the Nodal-inhibited EpiSCs resembles that of the anterior epiblast of embryonic day (E)7.0 and E7.5 mouse embryo, which is accompanied by chromatin modifications that reflect the priming of ectoderm lineage-related genes for expression. Nodal-inhibited EpiSCs show enhanced ectoderm differentiation in vitro and contribute to the neuroectoderm and the surface ectoderm in postimplantation chimeras but lose the propensity for mesendoderm differentiation in vitro and in chimeras. Our findings show that specification of the ectoderm progenitors is enhanced by the repression of Nodal signaling activity, and the ectoderm-like stem cells provide an experimental model to investigate the molecular characters of the epiblast-derived ectoderm.
学科主题Cell Biology
WOS关键词MOUSE EMBRYO ; VISCERAL ENDODERM ; AXIS FORMATION ; PRIMITIVE STREAK ; RNA-SEQ ; GASTRULATION ; PLURIPOTENCY ; REQUIREMENT ; EMBRYOGENESIS ; MORPHOGENESIS
语种英语
WOS记录号WOS:000438067600006
出版者CELL PRESS
版本出版稿
源URL[http://202.127.25.144/handle/331004/725]  
专题中国科学院上海生命科学研究院营养科学研究所
作者单位1.Childrens Med Res Inst, Bioinformat Grp, Westmead, NSW 2145, Australia;
2.Univ Sydney, Sydney Med Sch, Sch Med Sci, Westmead, NSW 2145, Australia,
3.Univ Chinese Acad Sci, Chinese Acad Sci, Shanghai Inst Biochem & Cell Biol, State Key Lab Cell Biol,CAS Ctr Excellence Mol Ce, 320 Yue Yang Rd, Shanghai 200031, Peoples R China;
4.CAS MPG Partner Inst Computat Biol, CAS Key Lab Computat Biol, Shanghai 200031, Peoples R China;
5.Childrens Med Res Inst, Embryol Unit, Westmead, NSW 2145, Australia;
6.ShanghaiTech Univ, Sch Life Sci & Technol, 100 Haike Rd, Shanghai 201210, Peoples R China;
推荐引用方式
GB/T 7714		 Liu, Chang,Wang, Ran,Yang, Xianfa,et al. Suppressing Nodal Signaling Activity Predisposes Ectodermal Differentiation of Epiblast Stem Cells[J]. STEM CELL REPORTS,2018,11(1):43-57.
APA Liu, Chang.,Wang, Ran.,Yang, Xianfa.,Chen, Jun.,Cui, Guizhong.,...&,.(2018).Suppressing Nodal Signaling Activity Predisposes Ectodermal Differentiation of Epiblast Stem Cells.STEM CELL REPORTS,11(1),43-57.
MLA Liu, Chang,et al."Suppressing Nodal Signaling Activity Predisposes Ectodermal Differentiation of Epiblast Stem Cells".STEM CELL REPORTS 11.1(2018):43-57.

入库方式： OAI收割

来源：上海营养与健康研究所

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